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US 9,791,372 B2Granted
Multiplexing and quantification in PCR with reduced hardware and requirements
AssigneeCalifornia Institute of Technology
InventorsImran R. Malik, Xiomara Linnette Madero, Erika F. Garcia, Sheel Mukesh Shah, Axel Scherer
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Abstract
Methods and algorithms for a multiplexed single detection channel amplification process and quantification of generated amplicons is presented. Various mathematical approaches for quantifying and verifying the amplicons in a reaction are presented. Usage of such methods and approaches allow upgrading of existing single and multiple channel instruments for further multiplexing capabilities.
Claims32
A method comprising:
- detecting, using a single channel detector, a plurality of different amplicons generated during a multiplexed amplification reaction,
- based on the detecting, generating a sum amplitude signal in correspondence of the plurality of different amplicons;
- quantifying, using a processor-based analyzer, the plurality of detected different amplicons, and
- verifying, using the processor-based analyzer, the plurality of detected different amplicons,
- wherein the quantifying and the verifying comprise using mathematical analysis of the generated sum amplitude signal, the mathematical analysis being implemented in one or combination of a software code and a firmware code and executed on the processor-based analyzer.
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Dependencies are inferred from the claim text.