Nanosplasmonic imaging technique for the spatio-temporal mapping of single cell secretions in real time
AssigneeThe United States of America as represented by the Secretary of the Navy
InventorsMarc P. Raphael, Joseph A. Christodoulides, Jeff M. Byers, James B. Delehanty
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Abstract
A label-free method for the spatio-temporal mapping of protein secretions from individual cells in real time by using a chip for localized surface plasmon resonance (LSPR) imaging. The chip is a glass coverslip compatible for use in a standard microscope having at least one array of functionalized plasmonic nanostructures patterned onto it. After placing a cell on the chip, the secretions from the cell are spatially and temporally mapped using LSPR imaging. Transmitted light imaging and/or fluorescence imaging may be done simultaneously with the LSPR imaging.
Claims6
A label-free method for the spatio-temporal mapping of secretions of anti-c-myc antibodies from individual cells in real time, comprising:
- using a chip for localized surface plasmon resonance (LSPR) imaging, comprising a glass coverslip compatible for use in a standard microscope and at least one array of functionalized plasmonic nanostructures patterned onto the glass coverslip, wherein the nanostructures were functionalized with a self-assembled monolayer comprising SH—(CH2)8-EG3-OH and SH—(CH2)11-EG3-NH2, and wherein the SH—(CH2)11-EG3-NH2 component was covalently conjugated with a c-myc peptide;
- placing at least one cell on the chip;
- and
- spatially and temporally mapping secretions of anti-c-myc antibodies from the cell using LSPR imaging.
Dependencies are inferred from the claim text.