Reaction vessel for sample preparation

A method of preparing purified and/or enriched bio-organic compounds from cellular material, viruses and/or sub-components of said cellular material and/or viruses, said method comprising

• (a) introducing said cellular material, viruses and/or sub-components of said cellular material and/or viruses, comprising proteins, into a reaction chamber of a container, said container further comprising a chromatographic medium and a receiving chamber, said receiving chamber being adjacent to said chromatography medium, said chromatographic medium separating said reaction chamber from said receiving chamber, wherein an outer face of said receiving chamber is closed and configured to be opened for obtaining said purified and/or enriched bio-organic compounds;
• (b) disrupting said cellular material, viruses and/or sub-components of said cellular material and/or viruses inside said reaction chamber;
• (c) performing proteolytic digestion;
• (d) eluting said bio-organic compounds,
• thereby obtaining said purified and/or enriched bio-organic compounds in said receiving chamber;
• wherein the bio-organic compounds comprise at least one of peptides, polypeptides, nucleic acids, lipids, or metabolites;
• wherein said disrupting is effected by
• (i) sonication;
• (ii) boiling in the presence of a chaotropic agent and/or a denaturing agent;
• and/or
• (iii) bead milling;
• and
• wherein said method is exclusively performed in said container.