Back to results
US 9,790,548 B2Granted
Methods and devices for amplification of nucleic acid
AssigneeIntelligent BioSystems, Inc.
InventorsSteven Gordon, Daniel Stetson
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods and devices for amplifying nucleic acid, and, in particular, amplifying so as to generate products on a surface without the use of emulsions. In a preferred embodiment, a plurality of groups of amplified product are generated on the surface, each group positioned in different (typically predetermined) locations on said surface so as to create an array.
Claims6
A method of amplifying and sequencing nucleic acid, comprising:
- a) providing i) a population of different nucleic acid template molecules, ii) a plurality of first and second single stranded oligonucleotides immobilized on a glass surface, iii) amplification reagents, iv) sequencing reagents, and v) a plurality of first and second sequencing primers;
- b) hybridizing at least a portion of said population of nucleic acid template molecules to said plurality of first oligonucleotides immobilized on said glass surface;
- c) amplifying said nucleic acid template molecules so as to create a plurality of forward and reverse single stranded strands;
- d) sequencing said forward oligonucleotide strands with said first sequencing primers;
- and
- e) sequencing said reverse oligonucleotide strands with said second sequencing primers.
3 more elements
Dependencies are inferred from the claim text.