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US 9,790,547 B2Granted
Integrated microfluidic and solid state pyrosequencing systems
AssigneeAgency for Science, Technology and Research
InventorsSanket Goel, Min Gong, Abdur Rub Abdur Rahman, Shihui Foo
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Abstract
The invention provides for sequencing a nucleic acid molecule based on the detection of base incorporation by the release of pyrophosphate (PPi) using a new enzyme system comprising adenosine diphosphate (ADP)-glucose pyrophosphorylase (AGPase) and its substrate ADP-glucose.
Claims18
A pyrosequencing kit for identifying a base at a target position in a sample DNA sequence, the kit comprising:
- (a) a pyrosequencing reagent comprising a polymerase;
- (b) a pyrosequencing reagent comprising a nucleotide triphosphate;
- (c) a pyrosequencing reagent comprising a pyrophosphate to ATP converting enzyme comprising adenosine diphosphate (ADP)-glucose pyrophosphorylase (AGPase) and a substrate ADP-glucose;
- and
- (d) a pyrosequencing reagent comprising an ATP detecting enzyme,
- wherein one or more of the polymerase, the AGPase, the ADP-glucose and the ATP detecting enzyme is immobilized on a solid support.
3 more elements
Dependencies are inferred from the claim text.