Microfluidic chip, device and system for the generation of aqueous droplets in emulsion oil for nucleic acid amplification

A microfluidic chip for the generation of aqueous droplets for nucleic acid amplification, said microfluidic chip comprising:

• (a) a first reservoir for uptake of a hydrophobic liquid ending in a plurality of inlets, wherein each inlet of said plurality of inlets is fluidly connected to one of a plurality of reaction vessels via one of a plurality of first microfluidic channels, wherein each inlet of said plurality of inlets is fluidly connected to a different first microfluidic channel of said plurality of first microfluidic channels, and each different first microfluidic channel is connected to a different reaction vessel, and (b) a plurality of second reservoirs for uptake of a hydrophilic solution, wherein each second reservoir of said plurality of second reservoirs ends in a vertical inlet fluidly connected to one of a plurality of second microfluidic channels connecting each second reservoir with a different first microfluidic channel via at least one T-junction, wherein said first reservoir is located within a first level and each second reservoir is located within a second level, wherein said first level is located below said second level, and wherein said at least one T-junction is characterized in that the end of each second microfluidic channel is joined to a different first microfluidic channel perpendicularly.