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US 9,790,544 B2Granted
DDAO compounds as fluorescent reference standards
AssigneeLife Technologies Corporation
InventorsScott C. Benson, Cinna Monighetti, Sandy M. Koepf
Life of this patent
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Abstract
According to the present teachings, methods and compositions are provided that utilize at least one reference dye of formula (I): In some embodiments, a method comprises measuring a detection signal of a reporter dye and at least one reference dye of formula (I). In some embodiments, a composition comprises a reference dye of formula (1), a buffer, a selection of nucleotides and a protein.
Claims18
An amplification master mix composition comprising:
- a buffer, a selection of nucleotides, at least one protein moiety, and a reference dye, wherein the reference dye has a structure according to formula (I):
- wherein:
- each of R1 to R3 and R6 to R8 is independently —H, halogen, —CO2H, —CO2R, —SO3H, —SO3R, —CH2CO2H, —CH2CO2R, —CH2SO3H, —CH2SO3R, —CH2NH2, —CH2NHR, —NO2, C1-C6 alkyl, substituted C1-C6 alkyl, C1-C6 alkoxy, and substituted C1-C6 alkoxy, wherein R is C1-C6 alkyl, substituted C1-C6 alkyl, C1-C6 alkoxy, and substituted C1-C6 alkoxy;
- R4 and R5 taken separately are selected from C1-C6 alkyl, and C1-C6 substituted alkyl, or R4 and R5 taken together are selected from C3-C7 cycloalkyl, C4-C7 unsaturated cycloalkyl, C3-C7 substituted cycloalkyl, or C4-C7 substituted unsaturated cycloalkyl.
1 more element
A multiwell plate comprising an amplification master mix composition, wherein the composition comprises:
- a buffer, a selection of nucleotides, at least one protein moiety, and a reference dye, wherein the reference dye has a structure according to formula (I):
- wherein:
- each of R1 to R3 and R6 to R8 is independently —H, halogen, —CO2H, —CO2R, —SO3H, —SO3R, —CH2CO2H, —CH2CO2R, —CH2SO3H, —CH2SO3R, —CH2NH2, —CH2NHR, —NO2, C1-C6 alkyl, substituted C1-C6 alkyl, C1-C6 alkoxy, and substituted C1-C6 alkoxy, wherein R is C1-C6 alkyl, substituted C1-C6 alkyl, C1-C6 alkoxy, and substituted C1-C6 alkoxy;
- R4 and R5 taken separately are selected from C1-C6 alkyl, and C1-C6 substituted alkyl, or R4 and R5 taken together are selected from C3-C7 cycloalkyl, C4-C7 unsaturated cycloalkyl, C3-C7 substituted cycloalkyl, or C4-,C7 substituted unsaturated cycloalkyl.
1 more element
Dependencies are inferred from the claim text.