Methods and kits for 3′-end-tagging of RNA
AssigneeEpicentre Technologies Corporation
InventorsRamesh Vaidyanathan, Scott Kuersten, Ken Doyle
Life of this patent
Estimating the term…
Abstract
The present innovation provides methods and kits that enable rapid and efficient dual end-tagging of RNA to prepare libraries for analysis by applications such as next-generation RNA sequencing, qPCR, microarray analysis, or cloning. The methods do not require time-consuming and inefficient gel-purification steps that are common to methods known in the art. In addition, the present invention provides methods and kits for rapid, high-throughput enzymatic preparation of 5′-activated, 3′-blocked DNA oligonucleotides from standard, single-stranded DNA oligonucleotides.
Claims13
A kit for preparing a library of nucleic acid molecules from one or more RNA acceptor oligonucleotides comprising:
- a) one or more 5′-adenylated, 3′-blocked DNA oligonucleotides;
- b) a RNA ligase or RNA ligases;
- c) a ligation buffer;
- d) Tobacco Acid Pyrophosphatase (TAP) or Yeast 5′-Deadenylase;
- e) RecJ Exonuclease or Lambda Exonuclease;
- and
- f) a reaction buffer.
Dependencies are inferred from the claim text.