Disposable container for bioburden sample collection and detection
AssigneeGeneral Electric Company
InventorsJin Huang, Weimin Xiao, Yan Huang, Chen Luo, Xiaoping Huang, Shukuan Xu, Wenwen Feng, Yan Jin
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Abstract
A disposable container for collection of microbe containing fluid samples is useful in a variety of microbial detection methods. One end of the container is provided with a lid comprising fluid flow apertures on one surface and a membrane filter (MF) on an opposed surface. Methods are disclosed wherein the container is disposed in a holding assembly with the lid gripped in a separate platform. The assembly and platform can be displaced relative to each other to separate the lid from the container.
Claims14
A method of detecting microbial presence in a fluid sample, the method comprising:
- filling at least a portion of a collection bottle with a fluid sample containing analyte microbes therein, said collection bottle comprising opposed top and bottom openings with at least one of said openings being sealed during said filling step;
- providing a first cap member on one opening of said openings, said first cap member comprising a first surface comprising fluid flow apertures therein and a membrane filter (MF) layer underlying said first surface;
- providing a second cap member on another opening of said openings, said second cap member comprising a fluid impervious needle penetrable surface;
- draining said fluid sample through said first cap member thereby depositing said analyte microbes on said MF layer;
- injecting a reagent into said fluid sample through said fluid impervious needle penetrable surface, said reagent configured to react with said analyte microbes to form a detectable presence on said MF layer, said reagent comprising a staining reagent or a luminescent agent configured to yield luminescent microbes on said MF layer;
- and
- placing a detector in operative association with said MF layer to detect said analyte microbes.
A method of detecting microbial presence in a fluid sample, the method comprising:
- filling at least a portion of a collection bottle with a fluid sample containing analyte microbes therein, said collection bottle comprising opposed top and bottom openings with at least one of said openings being sealed during said filling step;
- providing a first cap member on one opening of said openings, said first cap member comprising a first surface comprising fluid flow apertures therein and a membrane filter (MF) layer underlying said first surface;
- providing a second cap member on another opening of said openings, said second cap member comprising a fluid impervious needle penetrable surface;
- draining said fluid sample through said first cap member thereby depositing said analyte microbes on said MF layer;
- and
- placing a detector in operative association with said MF layer to detect said analyte microbes.
Dependencies are inferred from the claim text.