Enzymes and polymerases for the synthesis of RNA
AssigneeModernaTX, Inc.
InventorsYuxun Wang, Divakar Ramakrishnan, Antonin De Fougerolles, Susan Whoriskey
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Abstract
The invention relates to compositions and methods for the design, evolution, preparation, and/or manufacture of enzymes for use with polynucleotides, primary transcripts and mmRNA molecules.
Claims15
An isolated polynucleotide encoding a chimeric enzyme for synthesizing a capped RNA molecule that comprises at least one chemical modification, the chimeric enzyme comprising (a) at least one capping enzyme that comprises a Vaccinia capping enzyme catalytic subunit (D1) and a Vaccinia capping enzyme stimulation subunit (D12), and (b) at least one T7 RNA polymerase (T7), in one of the following combinations:
- (i) a single polypeptide comprising D1 linked to D12 linked to T7;
- (ii) a single polypeptide comprising D12 linked to D1 linked to T7;
- (iii) a single polypeptide comprising T7 linked to D1 linked to D12;
- or
- (iv) a single polypeptide comprising T7 linked to D12 linked to D1.
Dependencies are inferred from the claim text.