Methods for enhancing the degradation or conversion of cellulosic material

A method for producing a fermentation product, comprising:

• (a) saccharifying a cellulosic material under high temperature conditions with an enzyme composition comprising a GH61 polypeptide having cellulolytic enhancing activity;
• (b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product;
• and (c) recovering the fermentation product from the fermentation;
• wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from the group consisting of:
• (a) a GH61 polypeptide having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
• 2;
• (b) a GH61 polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
• 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.;
• (c) a GH61 polypeptide encoded by a polynucleotide having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 1 or the cDNA sequence thereof;
• and
• (d) a fragment of the GH61 polypeptide of (a), (b), or (c) that has cellulolytic enhancing activity;
• wherein the high temperature conditions are a temperature of about 54° C. to about 70° C. for about 6 to about 168 hours at a pH of about 3 to about 8 and a dry solids content of a cellulosic material of about 5 to about 50 wt %;
• wherein an effective amount of the GH61 polypeptide is about 0.01 to about 50.0 mg per g of the cellulosic material.