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US 9,790,526 B2Granted
CDNA encoding enone oxidoreductase from mango
AssigneeCouncil of Scientific and Industrial Research
InventorsVidya Shrikant Gupta, Ram Shridhar Kulkarni, Ashok Prabhakar Giri, Keshav H. Pujari
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Abstract
Disclosed herein are primers for amplifying enone oxidoreductase, having a sequence selected from the group consisting of SEQ ID Nos. 1 to 13, from mango. Also disclosed herein is a nucleotide sequence of SEQ ID No. 14 encoding enone oxidoreductase, for enzyme production in an artificial system thus generating the desired flavor in food products.
Claims5
A bacterial host cell comprising a vector comprising a promoter operably linked to a cDNA comprising SEQ ID NO:
- 14.
A method for semi-biosynthesis of flavors, comprising:
- expressing in vitro a cDNA comprising SEQ ID NO:
- 14 so as to yield an enone oxidoreductase;
- isolating the enone oxidoreductase;
- and
- contacting the isolated enone oxidoreductase with a substrate therefor under conditions for producing a mixture having a flavor as a result of conversion of the substrate to 4-hydroxy-2,5-dimethyl-3(2H)-furanone) by the enone oxidoreductase, wherein the conditions include the presence of a co-factor.
2 more elements
Dependencies are inferred from the claim text.