Modified serotype 28 adenoviral vectors

A replication-deficient serotype 28 adenoviral vector comprising chimeric hexon and fiber proteins selected from the group consisting of:

• (a) a chimeric hexon protein comprising a portion of an adenovirus serotype 45 hexon protein in place of a corresponding portion of the endogenous serotype 28 hexon protein, wherein the portion of adenovirus serotype 45 hexon protein consists of the amino acid sequence of SEQ ID NO:
• 1 or an amino acid sequence that is at least 95% identical to SEQ ID NO:
• 1;
• (b) a chimeric fiber protein comprising a portion of an adenovirus serotype 45 fiber protein in place of a corresponding portion of the endogenous serotype 28 fiber protein, wherein the portion of an adenovirus serotype 45 fiber protein consists of the amino acid sequence of SEQ ID NO:
• 3 or an amino acid sequence that is at least 95% identical to SEQ ID NO:
• 3;
• and
• (c) the chimeric hexon protein of (a) and the chimeric fiber protein of (b), wherein the adenoviral vector requires complementation of a deficiency in one or more early regions of the adenoviral genome for propagation and does not require complementation of any other deficiency of the adenoviral genome for propagation.