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US 9,790,518 B2Granted
Furin-knockdown and GM-CSF-augmented (FANG) cancer vaccine
AssigneeGradalis, Inc.
InventorsJohn J. Nemunaitis, Neil Senzer, Phillip B. Maples, Donald Rao
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Abstract
Compositions and methods for cancer treatment are disclosed herein. More specifically, the present invention describes an autologous cancer vaccine genetically modified for Furin knockdown and GM-CSF expression. The vaccine described herein attenuates the immunosuppressive activity of TGF-β through the use of bi-functional shRNAs to knock down the expression of furin in cancer cells, and to segment tumor antigen expression, presentation, and processing through expression of the GM-CSF transgene.
Claims11
A method of treating a cancer in an individual in need thereof comprising:
- a. transfecting an autologous tumor cell from the individual with an expression vector comprising:
- i. a first insert comprising a nucleic acid sequence encoding a Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) sequence;
- and
- ii. a second insert comprising a sequence according to SEQ ID NO:
- 2;
- and
- b. administering the transfected tumor cell to the individual.
4 more elements
Dependencies are inferred from the claim text.