Intergenic elements for enhancing gene expression
AssigneeCellaGenics B.V.
InventorsArie Pieter Otte, Michel Siep, John Antonius Verhees, Femke Hoeksema, Henricus Johannes Maria Van Blokland
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Abstract
The present invention relates to nucleic acid fragments and constructs comprising genomic nucleotide sequences, which are present upstream of Rb1 and p15C that are associated with intergenic transcription, for the production of a gene product of interest in a eukaryotic, preferably mammalian, host cell in the presence of a stringent selectable marker. The invention further relates to host cells comprising the nucleic acid constructs, to methods for generating the host cells and to methods for producing a gene product of interest using the host cells.
Claims15
A nucleic acid construct comprising:
- (i) a first nucleic acid fragment having:
- (a) at least 98% nucleotide sequence identity over its entire length with a fragment consisting of nucleotide residues 1-1019, 1-1482, 1-2018, 1-3498, 479-1482 or 479-2018 of SEQ ID NO:
- 5;
- (b) at least 98% nucleotide sequence identity over its entire length with a fragment consisting of nucleotide residues 1-2425, 1-2448, 1-3424 or 2425-3424 of SEQ ID NO:
- 6;
- or
- (c) at least 98% nucleotide sequence identity over its entire length with a fragment consisting of nucleotide residues 1-3064, 1-2500 or 1-2000 of SEQ ID NO:
- 7, and
- (ii) a nucleotide sequence encoding a selectable marker, which provides resistance against lethal or growth-inhibitory effects of a selection agent or wherein the marker complements a growth-inhibitory deficiency in the cell.
Dependencies are inferred from the claim text.