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US 9,790,515 B2Granted
Mutated allene oxide synthase 2 (AOS2) genes
AssigneesCibus US LLC, Cibus Europe B.V.
InventorsUvini Gunawardena, Gregory F. W. Gocal, Peter R. Beetham, Keith A. Walker
Life of this patent
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Abstract
Provided are compositions and methods relating to gene and/or protein mutations in plants. In certain embodiments, the disclosure relates to mutations in the allene oxide synthase 2 gene (i.e., AOS2). In some embodiments the disclosure relates to plants that are pathogen resistant.
Claims17
A method for producing a non-transgenic plant cell with a mutated AOS2 gene, comprising
- introducing into a plant cell a gene repair oligonucleobase (GRON) with a targeted mutation in an allene oxide synthase (AOS2) gene to produce a plant cell with an AOS2 gene that expresses an AOS2 protein comprising a G/T substitution at an amino acid position corresponding to G231 of SEQ ID NO:
- 5, wherein the G/T substitution is encoded by the codon ACT.
Dependencies are inferred from the claim text.