Yeast strains for the production of biomass on a substrate comprising a C5 sugar
AssigneeLesaffre Et Compagnie
InventorsThomas Desfougeres, Georges Pignede
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Abstract
The present invention concerns novel Saccharomyces cerevisiae yeast strains capable of multiplying on a substrate comprising at least one C5 sugar with a speed and rate of multiplication compatible with the industrial production of yeast. It also concerns novel strains which, when cultured, make it possible to obtain yeasts having an application efficiency, i.e. an efficiency that is satisfactory in applications and uses of interest in industries such as breadmaking, biomass production, flavour production, the production of secondary metabolites, protein production, ethanol production, brewing, winemaking or the production of yeast extract.
Claims11
A yeast strain of the saccharomyces genus, capable of multiplying under aerobic conditions on a substrate comprising at least one C5 sugar, wherein said strain satisfies a growth test on xylose or has a maximum speed of multiplication such that it satisfies a growth test on CP medium, and wherein, in its genome
- the expression of genes RPE1, RKI1, TKL1 and TAL1 of the pentose pathway has been placed under the control of a promoter of a gene not repressed by anaerobiosis or by the catabolic repression induced by any carbon source, and expressed during alcohol fermentation;
- at least one copy of the GRE 3 gene coding for an aldose reductase is deleted;
- the native XKS1 gene has been overexpressed;
- at least one copy of a Pichia Stipitis gene coding for a xylitol dehydrogenase has been introduced;
- and at least one copy of a gene coding for a xylose isomerase has been introduced.
Dependencies are inferred from the claim text.