Method to improve Lactococcus preservation
AssigneeIntrexon Actobiotics NV
InventorsErik Remaut, Dirk Iserentant
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Abstract
Lactococcus lactis strains with improved preservation characteristics, and improved acid and bile salt tolerance are disclosed. More specifically, a L. lactis strain having a heterologous trehalose-6-phosphate synthase gene and/or a trehalose-6-phosphate phosphatase gene, results in an accumulation of trehalose in the cytoplasm and/or in the cytoplasmic membrane. A L. lactis strain, having an internal trehalose concentration of at least 10 mg per gram cells (w/w) is disclosed.
Claims18
An isolated Lactococcus lactis strain comprising an internal trehalose concentration of at least 10 mg per gram wet weight of the L. lactis cells, wherein the strain expresses a heterologous trehalose-6-phosphate phosphatase gene, and wherein trehalose-6-phosphate phosphorylase activity is inactivated in the L. lactis cells.
Dependencies are inferred from the claim text.