Peanut-binding nucleic acid molecule and use thereof
AssigneeNEC Solution Innovators, Ltd.
InventorsTomoko Murata, Katsunori Horii, Ikuo Shiratori, Jou Akitomi, Naoto Kaneko, Iwao Waga
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Abstract
The present invention provides a novel nucleic acid molecule that can be used for detection of peanuts. The peanut-binding nucleic acid molecule of the present invention is characterized in that it binds to a peanut allergen with a dissociation constant of 10 nM or less, and preferably contains a polynucleotide consisting of any of base sequences of SEQ ID NOs: 1 to 15, for example. It is also preferable that, for example, the peanut-binding nucleic acid molecule of the present invention binds with significant specificity to the peanut allergen rather than to a soybean protein.
Claims10
A peanut-binding nucleic acid molecule, comprising at least one polynucleotide selected from the group consisting of the following polynucleotides (a) to (b):
- (a) a polynucleotide consisting of any of base sequences of SEQ ID NOs:
- 1 to 4, 6-10 and 12-14;
- and
- (b) a polynucleotide that consists of a base sequence with an identity of at least 80% to any of the base sequences of the polynucleotide (a) and binds to a peanut allergen.
A peanut detection sensor comprising:
- a peanut-binding nucleic acid molecule;
- and
- a nucleic acid molecule that forms a G-quartet structure, wherein the peanut-binding nucleic acid molecule comprises at least one of the following polynucleotides (a′) and (b′):
- (a′) a polynucleotide consisting of any of base sequences of SEQ ID NOs:
- 1 to 15;
- and
- (b′) a polynucleotide that consists of a base sequence with an identity of at least 80% to any of the base sequences of the polynucleotide (a′) and binds to a peanut allergen.
Dependencies are inferred from the claim text.