Interfering RNA molecules
AssigneeSilence Therapeutics GmbH
InventorsKlaus Giese, Jörg Kaufmann, Anke Klippel-Giese
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Abstract
The present invention is related to a ribonucleic acid comprising a double stranded structure whereby the double-stranded structure comprises a first strand and a second strand, whereby the first strand comprises a first stretch of contiguous nucleotides and whereby said first stretch is at least partially complementary to a target nucleic acid, and the second strand comprises a second stretch of contiguous nucleotides whereby said second stretch is at least partially identical to a target nucleic acid, and whereby the double stranded structure is blunt ended.
Claims30
A double-stranded siRNA molecule against a target nucleic acid, wherein the double-stranded siRNA molecule comprises a first strand and a second strand,
- wherein the first strand comprises a first stretch that is complementary to the target nucleic acid,
- wherein the second strand comprises a second stretch that is the same length as the first stretch and is complementary to the first stretch,
- wherein the first strand and the second strand form a double-stranded structure comprising the first stretch and the second stretch,
- wherein the first stretch and the second stretch each comprises contiguous alternating 2′-O-alkyl ribonucleotides, wherein the 2′-O-alkyl ribonucleotides alternate with unmodified or differently modified ribonucleotides,
- wherein the 2′-O-alkyl ribonucleotides of the first strand are shifted by one ribonucleotide relative to the 2′-O-alkyl ribonucleotides of the second strand, and
- wherein each of the first stretch and the second stretch consists of at least 17 and fewer than 30 ribonucleotides.
Dependencies are inferred from the claim text.