Method for producing novel hiPSC by means of miRNA introduction
AssigneeNational University Corporation Tottori University
InventorNorimasa Miura
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Abstract
A novel compound to induce a pluripotent stem cell is provided. A novel anti-malignant-tumor substance is provided. A pluripotent stem cell-inducing agent, including a single-stranded or double-stranded polynucleotide containing the base sequence shown in SEQ ID NO:41 or a base sequence including deletion, substitution, or addition of 1 to 3 bases in SEQ ID No: 41, in which the pluripotent stem cell-inducing agent induces a cell to become a pluripotent stem cell is provided.
Claims20
A method for producing a stem cell that differentiates into osteoblast cell, liver cell or fat cell, comprising introducing into a hepatoma cell or a fibrosarcoma cell a polynucleotide containing a base sequence shown in SEQ ID NO:41 or a base sequence including deletion, substitution, or addition of 1 to 3 bases in SEQ ID No:
- 41, and detecting expression of an undifferentiation marker of the cell or culturing the cell in a medium for ES cells.
A method for producing a stem cell that differentiates into osteoblast cell, liver cell or fat cell, comprising introducing into a hepatoma cell or a fibrosarcoma cell a vector containing a base sequence encoding a polynucleotide containing a base sequence shown in SEQ ID NO:41 or a base sequence including deletion, substitution, or addition of 1 to 3 bases in SEQ ID No:
- 41, and detecting expression of an undifferentiation marker of the cell or culturing the cell in a medium for ES cells.
Dependencies are inferred from the claim text.