Methods and compositions for segregating target nucleic acid from mixed nucleic acid samples
AssigneeFLIR Detection, Inc.
InventorRoger Allyn Forsyth
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Abstract
The invention provides methods, compositions and kits for segregating a target nucleic acid from a mixed nucleic acid sample. The methods, compositions and kits comprise a non-processive endonuclease (e.g., a restriction enzyme) or an antibody that binds the target nucleic acid (e.g., has methylation specificity). The mixed nucleic acid sample can comprise prokaryotic and eukaryotic nucleic acid and/or nucleic acid from more than one prokaryotic or eukaryotic organisms.
Claims24
A composition for segregating a target nucleic acid from a mixed sample containing a target nucleic acid and a non-target nucleic acid, comprising:
- (i) a non-processive endonuclease that binds to the target nucleic acid, but does not cleave the target nucleic acid;
- and
- (ii) a buffer having conditions suitable for the non-processive endonuclease to bind the target nucleic acid, but not cleave the target nucleic acid;
- wherein the buffer contains EDTA at a concentration of at least 10 mM.
A composition for segregating a target nucleic acid from a mixed sample containing a target nucleic acid and a non-target nucleic acid, comprising:
- (i) a non-processive endonuclease that binds to the target nucleic acid, but does not cleave the target nucleic acid;
- and
- (ii) a buffer having conditions suitable for the non-processive endonuclease to bind the target nucleic acid, but not cleave the target nucleic acid;
- wherein the non-processive endonuclease is a non-processive restriction enzyme.
A composition for segregating a target nucleic acid from a mixed sample containing a target nucleic acid and a non-target nucleic acid, comprising:
- (i) a non-processive endonuclease that binds to the target nucleic acid, but does not cleave the target nucleic acid;
- and
- (ii) a buffer having conditions suitable for the non-processive endonuclease to bind the target nucleic acid, but not cleave the target nucleic acid;
- wherein the non-processive endonuclease is DpnI.
Dependencies are inferred from the claim text.