Therapeutic nuclease compositions and methods
AssigneeUniversity of Washington
InventorsJeffrey A. Ledbetter, Martha Hayden-Ledbetter, Keith Elkon, Xizhang Sun
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Abstract
Hybrid nuclease molecules and methods for treating an immune-related disease or disorder in a mammal, and a pharmaceutical composition for treating an immune-related disease in a mammal.
Claims84
A nucleic acid comprising a nucleotide sequence encoding a hybrid nuclease molecule comprising human RNase 1 operatively coupled with or without a linker to an Fc domain, and human DNase I operatively coupled with or without a linker to the Fc domain, wherein the Fc domain is a human immunoglobulin Fc domain or a mutant human immunoglobulin Fc domain.
A nucleic acid comprising a nucleotide sequence encoding a hybrid nuclease molecule comprising (a) the amino acid sequence set forth in SEQ ID NO:
- 151, with or without a leader sequence, or (b) the amino acid sequence set forth in SEQ ID NO:
- 151, with or without a leader sequence and comprising one or more Fc mutations selected from P238S, P331S, K322S, and N297S, numbering is according to the EU index.
A nucleic acid comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 152, with or without a leader sequence.
A nucleic acid comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 153, with or without a leader sequence.
A nucleic acid comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 173, with or without a leader sequence.
A nucleic acid comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 177, with or without a leader sequence.
A nucleic acid comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 179, with or without a leader sequence.
A nucleic acid comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 181, with or without a leader sequence.
A nucleic acid comprising a nucleotide sequence encoding a human RNase 1 operatively linked with or without a linker to the N-terminus of a mutant human IgG1 Fc domain, and human DNase I operatively linked with or without a linker to the C-terminus of the mutant Fc domain, wherein the human RNase 1 comprises a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 149, with or without a leader sequence, and wherein the human DNase I comprises a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO:
- 139.
Dependencies are inferred from the claim text.