Transposon vector for vertebrate and invertebrate genetic manipulation
AssigneeThe Regents of the University of California
InventorsPeter W. Atkinson, Susan R. Wessler, Kun Liu
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Abstract
The present invention provides a transposon for use in genetic manipulation of vertebrate and invertebrate cells.
Claims12
A recombinant expression vector comprising a polynucleotide encoding a Muta1 transposase having at least 95% amino acid sequence identity to SEQ ID NO:2.
A recombinant vector comprising a heterologous nucleic acid comprising a Muta1 transposon, wherein the Muta1 transposon comprises a 5′ inverted terminal repeat comprising the nucleotide sequence of SEQ ID NO:3 and a 3′ inverted terminal repeat comprising the nucleotide sequence of SEQ ID NO:4, wherein the 5′ and 3′ inverted terminal repeats flank a nucleic acid sequence to be inserted into a target polynucleotide.
A gene transfer system comprising:
- a recombinant expression vector comprising a polynucleotide encoding a Muta1 transposase having at least 95% amino acid sequence identity to SEQ ID NO:2;
- and
- a nucleic acid comprising a Muta1 transposon, wherein the Muta1 transposon comprises a 5′ inverted terminal repeat comprising the nucleotide sequence of SEQ ID NO:3 and a 3′ inverted terminal repeat comprising the nucleotide sequence of SEQ ID NO:4, wherein the 5′ and 3′ inverted terminal repeats flank a nucleic acid sequence to be inserted into a target polynucleotide;
- and further, wherein the nucleic acid comprising the Muta1 transposon is contained in the recombinant expression vector comprising the polynucleotide encoding the Muta1 transposase or is contained in a separate recombinant vector.
A method of introducing a nucleic acid sequence of interest into a target polynucleotide, the method comprising introducing a Muta1 transposon nucleic acid into a host cell that comprises a heterologous Muta1 transposase comprising the amino acid sequence of SEQ ID NO:2;
- wherein the Muta1 transposon nucleic acid comprises a 5′ inverted terminal repeat comprising the nucleotide sequence of SEQ ID NO:3, a 3′ inverted terminal repeat comprising the nucleotide sequence of SEQ ID NO:4, and a nucleic acid sequence of interest to be inserted into the target polynucleotide, said nucleic acid sequence of interest flanked by the 5′ and 3′ inverted terminal repeat sequences.
Dependencies are inferred from the claim text.