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US 9,790,476 B2Granted
Modified transposases for improved insertion sequence bias and increased DNA input tolerance
AssigneeIllumina, Inc.
InventorsChristian Gloeckner, Amirali Kia, Erin Bomati, Molly He, Haiying Li Grunenwald, Scott Kuersten, Trina Faye Osothprarop, Darin Haskins, Joshua Burgess, Anupama Khanna, Daniel Schlingman, Ramesh Vaidyanathan
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Abstract
Presented herein are transposase enzymes and reaction conditions for improved fragmentation and tagging of nucleic acid samples, in particular altered transposases and reaction conditions which exhibit improved insertion sequence bias, as well as methods and kits using the same.
Claims20
A mutant Tn5 transposase having transposase activity, wherein said mutant Tn5 transposase comprises all of SEQ ID NO:
- 1 or SEQ ID NO:
- 11 except for:
- (a) mutations at one or more positions corresponding to positions 214 and 338 of SEQ ID NO:
- 1 or SEQ ID NO:
- 11, and optionally
- (b) mutations at one or more positions corresponding to positions 54, 56, 212, 251 and 372 of SEQ ID NO:
- 1 or SEQ ID NO:
- 11.
5 more elements
Dependencies are inferred from the claim text.