Method of detecting and/or identifying adeno-associated virus (AAV) sequences and isolating novel sequences identified thereby
AssigneeThe Trustees of the University of Pennsylvania
InventorsGuangping Gao, James M. Wilson, Mauricio R. Alvira
Life of this patent
Estimating the term…
Abstract
Adeno-associated virus rh.10 sequences, vectors containing same, and methods of use are provided.
Claims10
A recombinant adeno-associated virus (AAV) comprising an AAVrh.10 capsid comprising vp1proteins, vp2 proteins and vp3 proteins, the vp1 proteins having the sequence of amino acids 1 to 738 of SEQ ID NO:81 or a sequence at least 95% identical to the full length amino acid sequence of SEQ ID NO:
- 81, said recombinant AAV having packaged in the capsid a nucleic acid molecule comprising at least one AAV inverted terminal repeat (ITR) and a non-AAV nucleic acid sequence encoding a gene product operably linked to sequences which direct expression of the product in a host cell, wherein the gene product is a vascular endothelial growth factor (VEGF).
A recombinant adeno-associated virus (AAV) comprising an AAVrh.10 capsid comprising vp1 proteins, vp2 proteins and vp3 proteins, the vp1 proteins having the sequence of amino acids 1 to 738 of SEQ ID NO:81 or a sequence at least 95% identical to the full length amino acid sequence of SEQ ID NO:
- 81, said recombinant AAV having packaged in the capsid a nucleic acid molecule comprising at least one AAV inverted terminal repeat (ITR) and a non-AAV nucleic acid sequence encoding a gene product operably linked to sequences which direct expression of the product in a host cell, wherein the gene product is β-glucuronidase (GUSB) or alpha-1 antitrypsin (A1AT).
A recombinant adeno-associated virus (AAV) comprising an AAV capsid comprising an AAVrh.10 vp3 protein having the amino acid sequence of 204 to 738 of SEQ ID NO:
- 81 or a sequence at least 95% identical to the full length of amino acid sequence of 204 to 738 of SEQ ID NO:
- 81, said recombinant AAV having packaged in the capsid a nucleic acid molecule comprising at least one AAV inverted terminal repeat (ITR) and a non-AAV nucleic acid sequence encoding Factor VIII operably linked to sequences which direct expression thereof in a host cell.
A recombinant adeno-associated virus (AAV) comprising an AAV capsid comprising an AAVrh.10 vp3 protein having the amino acid sequence of 204 to 738 of SEQ ID NO:
- 81 or a sequence at least 95% identical to the full length amino acid sequence of 204 to 738 of SEQ ID NO:
- 81, said recombinant AAV having packaged in the capsid a nucleic acid molecule heterologous to AAV rh.10 comprising at least one AAV inverted terminal repeat (ITR) and a non-AAV nucleic acid sequence encoding Factor IX operably linked to sequences which direct expression thereof in a host cell.
A recombinant adeno-associated virus (AAV) comprising an AAV capsid comprising an AAVrh.10 vp3 having the amino acid sequence of 204 to 738 of SEQ ID NO:
- 81 or a sequence at least 95% identical to the full length amino acid sequence of 204 to 738 of SEQ ID NO:
- 81, said recombinant AAV having packaged in the capsid a nucleic acid molecule comprising at least one AAV inverted terminal repeat (ITR) and a non-AAV nucleic acid sequence which encodes erythropoietin operably linked to sequences which direct expression thereof in a host cell.
Dependencies are inferred from the claim text.