Cell culture medium
AssigneeBioMarker Kft.
InventorsHenriette Skribek, László Székely
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Abstract
The present invention relates to a cell culture medium for culturing human cells comprising an anti-coagulated total blood material wherein the hemoglobin level is from about 8 to about 16 g/dl. More particularly, the invention provides a cell culture medium in which cells present in the blood are disrupted and the insoluble remnants of the lysated cells are removed. Further, the invention provides a method for the preparation of a cell culture medium for culturing human cells, according to the invention.
Claims16
A method for culturing human cells, said method comprising
- preparing an anti-coagulated total blood material that lacks the membrane fraction of red blood cells and has a hemoglobin level from 8 to 16 g/dl by
- (a) obtaining mammalian blood having an extracellular fraction, as starting material;
- (b) adding an anticoagulant to the mammalian blood thereby providing anti-coagulated mammalian blood;
- (c) mechanically disrupting the cells present in the anti-coagulated mammalian blood;
- (d) removing insoluble remnants of the disrupted cells to produce a particle-free extract;
- (e) modifying the ion content of the particle-free extract to match the extracellular fraction of the starting material or to be similar to that of a medium selected form the group consisting of Dulbecco's Modified Eagle's Medium (DMEM), Roswell Park Memorial Institute (RPMI) medium and Isocove's Modified Dulbecco's Medium (IMDM), to produce a cell culture medium;
- and
- (f) culturing the human cells in the cell culture medium.
A method for culturing human cells, said method comprising
- preparing an anti-coagulated total blood material that lacks the membrane fraction of red blood cells by
- (a) obtaining mammalian blood having an extracellular fraction, as starting material;
- (b) adding an anticoagulant to the mammalian blood thereby providing anti-coagulated mammalian blood;
- (c) lysing the cells present in the anti-coagulated mammalian blood to produce lysed anti-coagulated mammalian blood;
- (d) removing insoluble remnants of the lysed cells from the lysed anti-coagulated mammalian blood;
- and
- (e) modifying the ion content of the lysed anti-coagulated mammalian blood to match that of the extracellular fraction of the starting material or to be similar to that of a medium selected form the group consisting of Dulbecco's Modified Eagle's Medium (DMEM), Roswell Park Memorial Institute (RPMI) medium and Isocove's Modified Dulbecco's Medium (IMDM),
- to produce a cell culture medium having a hemoglobin level from 8 to 16 g/d;
- and
- culturing the human cells in the cell culture medium.
Dependencies are inferred from the claim text.