US 9,790,288 B2Granted

Fractionation of charged polysaccharide

AssigneeLipoxen Technologies Limited

InventorsSanjay Jain, Ioannis Papaioannou, Peter Laing

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Abstract

Polydisperse and charged polysaccharides are fractionated into low polydispersity fractions (preferably having pd<1.1), each containing species within a narrow range of molecular weights. An aqueous solution of the polydisperse polysaccharides is contacted with an ion exchange resin in a column and the polysaccharides are subjected to selective elution by aqueous elution buffer. The selective elution consists of at least 3 sequential elution buffers having different and constant ionic strength and/or pH and in which the subsequent buffers have ionic strength and/or pH than those of the preceding step. The new preparations are particularly suitable for the production of PSA-derivatised therapeutic agents intended for use in humans and animals.

Claims30

Dependent claims

A process for separating a population of polydisperse polysialic acid compounds into fractions of different molecular weight and narrow polydispersity, the process comprising the steps of:

sequentially applying at least three elution buffers to an ion-exchange column previously contacted with a solution comprising the population of polydisperse polysialic acid compounds, the application of each of the at least three elution buffers resulting in a selective elution of a fraction of polysialic acid compounds, thereby separating the population of polydisperse polysialic acid compounds into fractions of different molecular weight and narrow polydispersity,
wherein each of the fractions comprise polysialic acid compounds having at least 10 sialic acid units and a polydispersity of less than 1.2,
wherein the at least three elution buffers include a first elution buffer, a second elution buffer and a third elution buffer, and
wherein the second elution buffer has a higher pH than the first elution buffer and the third elution buffer has a higher pH than the second elution buffer.

1 more element

Dependencies are inferred from the claim text.

Facts

Application: 14/924,637Filed 27 Oct 2015 · continuation
Priority: EP 0525101623 Feb 2005
Published: US 2016/0159936 A19 Jun 2016
Granted: 17 Oct 2017
Term ends: Not estimatedThe source records don’t support an estimate.

Classification14 codes

C08B 37/0006
Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof: homoglycans, i.e. polysaccharides having a…
A61K 47/61
The modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule: the organic macromolecular…
B01D 15/361
Involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion: ion-exchange
B01D 15/362
Ion-exchange: cation-exchange

Family

PCT/GB2004/003511Parent of this continuation-in-part · filed 12 Aug 2004
PCT/GB2005/003149Parent of this continuation · filed 12 Aug 2005
Application14/155,754Parent of this continuation · filed 15 Jan 2014
US 8,652,334 B2Parent of this continuation · filed 18 Feb 2014
US 2016/0159936 A1Pre-grant publication · published 9 Jun 2016
US 9,200,052 B2Parent of this continuation
Application11/660,133Parent of this continuation
Application11/660,133Parent of this continuation-in-part
US 2008/0132696 A1Published application
US 2014/0127779 A1Published application
US 9,790,288 B2This patent

From the US filing history. Foreign family members aren’t listed.