Glypican-3-specific antibody and uses thereof

An isolated and substantially purified polynucleotide encoding a single chain human anti-glypican-3 (GPC3) antibody fragment, wherein the single chain antibody fragment comprises a heavy chain variable region and light chain variable region, wherein the amino acid sequence of the heavy chain variable region and the amino acid sequence of the light chain variable region are selected from the group consisting of:

• (a) SEQ ID NO:
• 12 and SEQ ID NO:
• 17;
• (b) SEQ ID NO:
• 13 and SEQ ID NO:
• 18;
• (c) SEQ ID NO:
• 14 and SEQ ID NO:
• 19;
• and, (d) SEQ ID NO:
• 15 and SEQ ID NO:
• 20.