Method for increasing ETEC CS6 antigen presentation on cell surface and products obtainable thereof
AssigneeScandinavian Biopharma Holding AB
InventorsNils Carlin, Ann-Mari Svennerholm, Joshua Tobias
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Abstract
A method for increasing the presentation of ETEC CS6 antigen on cell surface, comprising the step of contacting cells expressing said antigen with an aqueous solution comprising 0.6-2.2 percent phenol by weight, such that the presentation of said antigen is increased by at least 100%. A method for the manufacture of a killed whole cell vaccine for immunization against CS6-expressing ETC. Cells and vaccines obtainable by the above methods.
Claims27
A phenol-treated cell having an amount of enterotoxigenic Escherichia coli “ETEC” Coli surface antigen 6 “CS6” antigen presented on a cell surface that is at least 100% greater than an untreated cell, wherein said 100% greater CS6 antigen presentation corresponds to at least 0.6 μg/109 bacteria.
A phenol treated cell having an amount of enterotoxigenic Escherichia coli “ETEC” Coli surface antigen 6 “CS6” antigen presented on a cell surface greater than an untreated cell, wherein the amount of surface CS6 antigen presented is at least 0.6 μg/109 bacteria, and wherein the cell was treated with 0.6 to 2.0% phenol by weight for a duration of 1 to 72 hours at a temperature of 18 to 42° C.
A phenol treated cell having an amount of enterotoxigenic Escherichia coli “ETEC” Coli surface antigen 6 “CS6” antigen presented on a cell surface greater than an untreated cell, wherein the amount of surface CS6 antigen presented is at least 0.6 μg/109 bacteria, and wherein the cell was treated at a temperature of 20±1° C. with:
- 0.6% phenol by weight for a duration of 40 hours;
- 0.8% phenol by weight for a duration of 16 hours;
- 0.8% phenol by weight for a duration of 40 hours;
- 1.0% phenol by weight for a duration of 6 hours;
- 1.0% phenol by weight for a duration of 16 hours;
- 1.0% phenol by weight for a duration of 40 hours;
- 1.2% phenol by weight for a duration of 6 hours;
- 1.2% phenol by weight for a duration of 16 hours;
- 1.2% phenol by weight for a duration of 40 hours;
- 1.5% phenol by weight for a duration of 6 hours;
- 1.5% phenol by weight for a duration of 16 hours;
- 1.5% phenol by weight for a duration of 40 hours;
- 2.0% phenol by weight for a duration of 6 hours;
- or
- 2.0% phenol by weight for a duration of 16 hours.
A phenol treated cell having an amount of enterotoxigenic Escherichia coli “ETEC” Coli surface antigen 6 “CS6” antigen presented on a cell surface greater than an untreated cell, wherein the amount of surface CS6 antigen presented is at least 0.6 μg/109 bacteria, and wherein the cell was treated with 1.0 to 1.5% phenol by weight for a duration of 6 to 40 hours at a temperature of 20±1° C.
Dependencies are inferred from the claim text.