Nucleic acid amplification reaction vessel and nucleic acid amplification reaction apparatus

A nucleic acid amplification reaction vessel comprising:

• a first inner wall;
• a second inner wall that is arranged opposite to the first inner wall, wherein a distance between the first inner wall and the second inner wall is a length shorter than a diameter b of a droplet of a nucleic acid amplification reaction solution, such that the droplet of the nucleic acid amplification reaction solution comes into contact with both the first inner wall and the second inner wall, simultaneously, when the reaction vessel is filled with a liquid and 1.0 μl to 7.0 μl of the nucleic acid amplification reaction solution is loaded into the reaction vessel, wherein the liquid has a lower specific gravity than the nucleic acid amplification reaction solution such that the reaction solution forms the droplet having the diameter b within the liquid;
• a third inner wall;
• and
• a fourth inner wall that is arranged opposite to the third inner wall, the third inner wall and fourth inner wall being orthogonal to the first inner wall and the second inner wall, wherein a distance between the third inner wall and the fourth inner wall is a length at least twice the diameter b of the droplet of the nucleic acid amplification reaction solution, such that the droplet of the nucleic acid amplification reaction solution does not come into contact with both the third inner wall and the fourth inner wall at the same time when the 1.0 μl to 7.0 μl of the nucleic acid amplification reaction solution is loaded into the reaction vessel filled with the liquid.