DNA sequence, and recombinant preparation of the grass pollen allergen Lol p 4
AssigneeMerck Patent GmbH
InventorsAndreas Nandy, Helmut Fiebig, Oliver Cromwell
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Abstract
The present invention relates to the provision of a DNA sequence of the major grass pollen allergen Lol p 4. The invention also encompasses fragments, new combinations of partial sequences and point mutants having a hypoallergenic action. The recombinant DNA molecules and the derived polypeptides, fragments, new combinations of partial sequences and variants can be utilized for the therapy of pollen-allergic diseases. The proteins prepared by recombinant methods can be employed for in vitro and in vivo diagnosis of pollen allergies.
Claims8
A DNA molecule encoding an Lol p 4 allergen having the amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO:
- 4, wherein the nucleotide sequence of the DNA molecule differs from SEQ ID:
- NO 1 and SEQ ID NO:
- 3, respectively, by at least once nucleotide, and wherein the nucleotide sequence has been codon optimized for the expression of said allergen polypeptide by recombinant methods in a host cell that is not a cell of Lolium perenne.
Dependencies are inferred from the claim text.