Method for the in vitro stabilization of glucose, lactate and homocysteine in a blood sample and method for determining the amount of blood components in a blood sample
AssigneePetra Weser-Bisse
InventorEmmanuel Bisse
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Abstract
The present invention relates to a composition for the stabilization of glucose, lactate and homocysteine in blood after collection, to a use of the provided compositions and a method for the stabilization of glucose, lactate and homocysteine in blood after collection, as well as optionally the in vitro determination of glucose, lactate and homocysteine in blood, and a blood collection device provided for said use and method.
Claims12
A method for stabilizing one or more of glucose, lactate and homocysteine in a blood sample in vitro, comprising the steps of
- a. providing a composition comprising in combination:
- i) at least one inhibitor of hexokinase, selected from the group consisting of 2-deoxy-D-glucose, 2-fluoro-2-deoxy-D-glucose, 2-amino-2-deoxy-D-glucose and 3-bromopyruvic acid or salt thereof with a concentration of at least 0.01 mg per mL blood;
- ii) at least one glycolysis-inhibiting agent having activity for another enzyme involved in glucose catabolism and having antiglycolytic activity for any one of the enzymes in the glycolytic pathway downstream of hexokinase, selected from the group consisting of fluoride salt, iodoacetic acid or salt thereof, oxamic acid or salt thereof and dichloroacetic acid or salt thereof with a concentration of at least 0.01 mg per mL blood;
- and
- iii) an anticoagulant and/or a plasma stabilizer, selected from the group consisting of EDTA salt, citrate salt, oxalate salt and heparin salt with a concentration of at least 0.01 mg per mL blood;
- and
- b. mixing the blood sample in vitro with the composition;
- wherein said blood sample is stabilized for further analysis.
A method of determining the amount of one or more blood components in a blood sample in vitro comprising the steps of:
- stabilizing the blood sample by mixing the blood sample with a composition comprising in combination:
- i) at least one inhibitor of hexokinase, selected from the group consisting of 2-deoxy-D-glucose, 2-fluoro-2-deoxy-D-glucose, 2-amino-2-deoxy-D-glucose and 3-bromopyruvic acid or salt thereof with a concentration of at least 0.01 mg per mL blood;
- ii) at least one glycolysis-inhibiting agent having activity for another enzyme involved in glucose catabolism and having antiglycolytic activity for any one of the enzymes in the glycolytic pathway downstream of hexokinase, selected from the group consisting of fluoride salt, iodoacetic acid or salt thereof, oxamic acid or salt thereof and dichloroacetic acid or salt thereof with a concentration of at least 0.01 mg per mL blood;
- and
- iii) an anticoagulant and/or a plasma stabilizer, selected from the group consisting of EDTA salt, citrate salt, oxalate salt and heparin salt with a concentration of at least 0.01 mg per mL blood, and
- determining the amount of one or more blood components in the stabilized blood sample.
Dependencies are inferred from the claim text.