Biocatalytic methods to convert cyclohexane oxidation process waste streams to useful products
AssigneeInvista North America S.A.R.L.
InventorsPaul S. Pearlman, Changlin Chen, Adriana Leonora Botes
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Abstract
The invention relates to methods for enriching monomer content in a cycloalkane oxidation process mixed organic waste stream. In particular, the methods involve combining a biocatalyst with a mixed organic waste stream from a cycloalkane oxidation process, and enzymatically converting dimeric and/or oligomeric components of said waste stream into monomeric components. The methods may enrich the content of diacids, adipic acid, and/or other α,ω-difunctional C6 alkanes in the mixed organic waste stream. Additionally, the treated mixed organic waste streams may have improved burning efficiency.
Claims32
A method of hydrolyzing dimeric, oligomeric, or cyclic esters in a cycloalkane oxidation process mixed organic waste stream, the method comprising
- contacting one or more isolated or extracellular recombinant enzymes having hydrolase activity with a mixed organic waste stream from a cycloalkane oxidation process;
- wherein the contacting results in hydrolysis of at least a portion of dimeric, oligomeric or cyclic esters of said waste stream into aliphatic monomeric components;
- and wherein
- said one or more recombinant enzymes having hydrolase activity comprises one or more hydrolases that are classified under EC 3.1.1.- and are selected from the group consisting of a lipase classified under EC 3.1.1.3, an esterase classified under EC 3.1.1.1, a cutinase classified under EC 3.1.1.74, a polyhydroxyalkanoate (PHA) depolymerase classified under EC 3.1.1.75 or EC 3.1.1.76, a lactone hydrolase classified under EC 3.1.1.25, and a gluconolactonase classified under EC 3.1.1.17.
A method to increase the C4-C6 diacids content of a cycloalkane oxidation process mixed organic waste stream, comprising:
- a. contacting said mixed organic waste stream with one or more isolated or extracellular recombinant hydrolases capable of hydrolyzing dimeric, oligomeric or cyclic esters such that at least a portion of dimeric, oligomeric or cyclic esters in said stream are hydrolyzed into linear C4-C6 aliphatic monomers, wherein said hydrolases are classified under EC 3.1.1.-;
- and
- b. contacting the mixed organic stream with a recombinant whole cell microbial biocatalyst having at least one exogenous nucleic acid encoding a polypeptide capable of oxidising linear C4-C6 mono-acids, hydroxy-acids and oxo-acids to corresponding diacids such that at least a portion of the linear C4-C6 mono-acids, hydroxy-acids and oxo-acids in said mixed organic waste stream are oxidized to the corresponding diacids.
A method to increase the adipic acid content of a cycloalkane oxidation process mixed organic waste stream, comprising:
- a. contacting said mixed organic waste stream with one or more isolated or extracellular recombinant hydrolases, wherein the contacting results in hydrolysis of at least a portion of the dimeric, oligomeric or cyclic esters in said waste stream into aliphatic monomeric components, wherein said hydrolases are classified under EC 3.1.1.-;
- b. contacting said mixed organic stream with a recombinant whole cell microbial biocatalyst capable of
- i. oxidising at least a portion of caproic acid, 6-hydroxycaproic acid and 6-oxocaproic acid present in said mixed organic waste stream to adipic acid;
- and
- ii. converting at least a portion of the cyclic C6 components in said mixed organic waste stream to adipic acid.
A method to convert C6 components present in a cycloalkane oxidation process mixed organic waste stream to α,ω-difunctional C6 alkanes, comprising
- a. contacting said mixed organic waste stream with one or more isolated or extracellular hydrolases capable of hydrolyzing dimeric, oligomeric or cyclic esters, wherein the contacting results in hydrolysis of at least a portion of the dimeric, oligomeric or cyclic esters present in said waste stream into aliphatic monomeric components, wherein said hydrolases are classified under EC 3.1.1.-;
- b. contacting the mixed organic stream with a recombinant whole cell microbial biocatalyst capable of
- i. oxidising at least a portion of caproic acid and 6-hydroxycaproic acid present in said mixed organic waste stream to 6-oxocaproic acid or adipic acid;
- ii. converting at least a portion of cyclic C6 components present in said mixed organic waste stream to 6-hydroxycaproic acid, 6-oxocaproic acid or adipic acid;
- and
- iii. expressing at least one heterologous enzyme that converts adipic acid, 6-hydroxycaproic acid or 6-oxohexanoic acid to 1,6-hexanediol, 6-aminocaproic acid, ε-caprolactam or hexamethylenediamine.
Dependencies are inferred from the claim text.