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US 9,782,468 B2Granted
Purification of polysaccharide protein conjugates
AssigneeShantha Biotechnics Private Limited
InventorsVijayarangam Damotharan, Sandeep Kumar Nettem, Raghavendra Maila
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Abstract
The invention describes a method of purifying polysaccharide protein conjugates using mixed mode chromatography. The method involves contacting a crude polysaccharide protein conjugate with a mixed mode resin comprising an inert porous shell and an activated core under conditions of low conductivity that allow binding of the contaminants and collecting the unbound polysaccharide protein conjugate in a flowthrough.
Claims25
A process of purifying a polysaccharide protein conjugate from one or more contaminants comprising the steps of:
- a. contacting a crude polysaccharide protein conjugate with a mixed mode resin comprising an inert porous shell and an activated core under conditions of low conductivity that allow binding of said contaminants, and
- b. collecting the unbound polysaccharide protein conjugate in a flowthrough,
- wherein a size of the unbound polysaccharide protein conjugate is greater than a molecular size cut-off of a pore size of the inert porous shell,
- wherein the polysaccharide protein conjugate is selected from the group consisting of Hib conjugate, meningococcal conjugate, pneumococcal conjugate and typhoid conjugate,
- wherein the inert porous shell comprises pores having a molecular weight cut off between 2000 kDa to 500 kDa, and
- wherein the method further comprises a dilution step and a diafiltration step before step (a).
3 more elements
Dependencies are inferred from the claim text.