Modified arsenite oxidase and a biosensor for detecting arsenite
AssigneeThe Bio Nano Centre Limited
InventorsAnthony Edward George Cass, Joanne Santini, Christopher James Johnson
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Abstract
The present invention provides an arsenite oxidase enzyme modified to prevent translocation by modification of a translocation signal sequence. A microorganism modified to express the heterologous arsenite oxidase enzyme is also provided by the invention, together with a device for detecting the presence of arsenite in a sample.
Claims5
An arsenite oxidase enzyme modified to prevent translocation to the periplasm, wherein the enzyme comprises [1]the native AioA subunit from NT-26, or a variant, homologue or derivative thereof having at least 90% sequence homology to the amino acid sequence of SEQ ID NO:4, and [2]the native AioB subunit from NT-26, or a variant, homologue or derivative thereof having at least 90% sequence homology to the amino acid sequence of SEQ ID NO:3, wherein a portion of the native AioB subunit corresponding to the translocation signal sequence or a functional fragment thereof, is modified, and wherein said enzyme has arsenite oxidase activity.
Dependencies are inferred from the claim text.