# US 9,775,314 B1 · Maize hybrid X90H616

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- Number: US 9,775,314 B1
- Document ID: US9775314B1
- Status: Granted
- Kind: B1
- Application number: 15/086,141
- Filed: 2016-03-31
- Granted: 2017-10-03
- Earliest claimed: 2016-03-31
- Assignees: Pioneer Hi-Bred International, Inc.
- Applicants: Pioneer Hi-Bred International, Inc.
- Inventors: Gustavo Marcelo Garcia
- Web page: https://patentgrep.com/patent/US9775314B1

## CPC

- A01H 6/4684: {Zea mays [maize]}
- A01H 5/10: Seeds
- C12N 15/8243: {involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine}
- C12N 15/8245: {involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis}
- C12N 15/8247: {involving modified lipid metabolism, e.g. seed oil composition}
- C12N 15/8251: {Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis}
- C12N 15/8274: {for herbicide resistance}
- C12N 15/8279: {for biotic stress resistance, pathogen resistance, disease resistance}
- C12N 15/8286: {for insect resistance}
- C12N 15/8289: {Male sterility}
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

A novel maize variety designated X90H616 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X90H616 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X90H616 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. This invention relates to the maize variety X90H616, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X90H616. This invention further relates to methods for producing maize varieties derived from maize variety X90H616.

## Claims

### Claim 1

A seed of hybrid maize variety X90H616, representative seed produced by crossing a first plant of variety PH2G6T with a second plant of variety PH1M77, wherein representative seed of the varieties PH2G6T and PH1M77 have been deposited under ATCC Accession Numbers PTA-124162 and PTA-122442, respectively.

### Claim 2 (depends on 1)

A plant of hybrid maize variety X90H616 grown from the seed of claim 1.

### Claim 3 (depends on 2)

A plant part or cell of the plant of claim 2.

### Claim 4 (depends on 1)

A method of producing the seed of claim 1, the method comprising crossing a plant of variety PH2G6T with a plant of variety PH1M77, wherein representative seed of varieties PH2G6T and PH1M77 have been deposited under ATCC Accession numbers PTA-124162 and PTA-122442, respectively.

### Claim 5

A seed of hybrid maize variety X90H616 further comprising at least a first transgene, representative seed of hybrid maize variety X90H616 produced by crossing a first plant of variety PH2G6T with a second plant of variety PH1M77, wherein representative seed of the varieties PH2G6T and PH1M77 have been deposited under ATCC Accession Numbers PTA-124162 and PTA-122442, respectively.

### Claim 6 (depends on 5)

The seed of claim 5, wherein the transgene confers a property selected from the group consisting of male sterility, herbicide tolerance, insect resistance, disease resistance, waxy starch, modified fatty acid metabolism, modified phytic acid metabolism, modified carbohydrate metabolism and modified protein metabolism.

### Claim 7 (depends on 5)

The hybrid maize variety X90H616 seed of claim 5, further comprising a seed treatment on the surface of the seed.

### Claim 8 (depends on 5)

A method for producing nucleic acids, the method comprising isolating nucleic acids from the hybrid maize variety X90H616 seed of claim 5.

### Claim 9 (depends on 5)

A plant grown from the seed of claim 5.

### Claim 10 (depends on 9)

A method for producing a second maize plant, the method comprising applying plant breeding techniques to the plant of claim 9 to produce the second maize plant.

### Claim 11 (depends on 9)

A method for producing a second maize plant, the method comprising doubling haploid seed generated from a cross of the maize plant of claim 9 with a different maize plant.

### Claim 12

A converted F1 hybrid maize variety X90H616 seed further comprising a locus conversion, the converted seed being produced by crossing a first plant of variety PH2G6T with a second plant of variety PH1M77; representative seed of the varieties PH2G6T and PH1M77 having been deposited under ATCC Accession Numbers PTA-124162 and PTA-122442, respectively; wherein at least one of the varieties PH2G6T and PH1M77 further comprises a locus conversion and wherein the converted seed produces a plant having otherwise the same phenotypic characteristics as maize variety X90H616 when grown under the same environmental conditions.

### Claim 13 (depends on 12)

The converted seed of claim 12, further comprising a seed treatment on the surface of the converted seed.

### Claim 14 (depends on 12)

The converted seed of claim 12, wherein the locus conversion confers a property selected from the group consisting of male sterility, herbicide tolerance, insect resistance, disease resistance, waxy starch, modified fatty acid metabolism, modified phytic acid metabolism, modified carbohydrate metabolism and modified protein metabolism.

### Claim 15 (depends on 12)

A method for producing nucleic acids, the method comprising isolating nucleic acids from the converted seed of claim 12.

### Claim 16 (depends on 12)

A plant, plant part, or plant cell produced by growing the converted seed of claim 12, the plant, plant part or plant cell comprising at least one converted F1 hybrid maize variety X90H616 cell.

### Claim 17 (depends on 16)

A method for producing nucleic acids, the method comprising isolating nucleic acids from the plant, plant part, or plant cell of claim 16.

### Claim 18 (depends on 16)

A method of producing a commodity plant product comprising starch, syrup, silage, fat or protein, the method comprising producing the commodity plant product from the plant or plant part of claim 16.

### Claim 19 (depends on 16)

A method for producing a second maize plant, the method comprising applying plant breeding techniques to the plant or plant part of claim 16 to produce the second maize plant.

### Claim 20 (depends on 16)

A method for producing a second maize plant, the method comprising doubling haploid seed generated from a cross of the maize plant or plant part of claim 16 with a different maize plant.