# US 9,775,299 B2 · Noninvasive method of source-sink regulation in rice

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- Number: US 9,775,299 B2
- Document ID: US9775299B2
- Status: Granted
- Kind: B2
- Application number: 14/441,955
- Filed: 2013-09-24
- Published: 2015-10-08
- Granted: 2017-10-03
- Earliest claimed: 2013-05-08
- Assignees: China National Rice Research Institute
- Applicants: China National Rice Research Institute
- Inventors: Weixing Zhang; Zhiwei Zhu; Xiyuan Liao; Xihong Shen; Guosheng Shao; Jie Min; Weigui Zhang
- Web page: https://patentgrep.com/patent/US9775299B2

## CPC

- A01G 22/00: Cultivation of specific crops or plants not otherwise provided for
- A01G 22/22: Rice
- A01H 1/022: {Genic fertility modification, e.g. apomixis}
- A01G: HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING (picking of fruits, vegetables, hops or the like A01D46/00; propagating unicellular algae C12N1/12)
- A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES

## Abstract

A noninvasive method of source-sink regulation in rice belongs to the technical field of rice production. In this method, the source-sink relationship is regulated by a rice sterile line and its identical type of maintaining line being subjected to mixed-planting and insulated pollination, or sowing and transplanting at different times and insulated pollination, so as to construct rice plant samples with gradient difference of source-sink levels. The present invention is a kind of native, natural noninvasive method of source-sink regulation, which could broaden the traditional thinking of source-sink theoretical research, especially overcome the deficiency in conventional methods such as leaf-cutting, spikelet-thinning that lead to physical injury or physiological interference. The method provides a brand new approach and solution for thoroughly investigating source-sink relationship in rice, wheat, maize and other crops, and will play an important role in enriching crop source-sink theory and also promoting the development of the related disciplines.

## Claims

### Claim 1

A noninvasive method of source-sink regulation in rice to construct different sink level differences, wherein the method comprises:
subjecting a rice sterile line (A) and its identical type of maintaining line (B) to mixed planting in a sterile:maintaining row ratio of 1:1, 2:1 or 4:2 or in a sterile:maintaining plant ratio of 6:3, sowing and transplanting, and insulated pollination;
wherein the sowing and transplanting comprise the sterile line (A) being sowed 3 days, 6 days, or 9 days earlier than its identical type of maintaining line (B), and the sterile line and its identical type of maintaining line being transplanted on the same day; and
wherein the rice sterile line and its identical type of maintaining line are selected from the group consisting of Zhong 9A/Zhong 9B and II-32A/II-32B.

### Claim 2 (depends on 1)

A noninvasive method of source-sink regulation in rice to construct different sink level differences of claim 1, wherein a plastic film is used for the insulated pollination.