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US 9,765,373 B2Granted
Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
AssigneesNovozymes A/S, Novozymes, Inc.
InventorsKirk Schnorr, Tarana Shaghasi, Matt Sweeney
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Abstract
The present invention relates to polypeptides having cellulolytic enhancing activity, catalytic domains, and carbohydrate binding domains, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding domains.
Claims48
A process for degrading a cellulosic material, comprising:
- treating the cellulosic material with an enzyme composition comprising a GH61 polypeptide having cellulolytic enhancing activity, wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from the group consisting of:
- (a) a GH61 polypeptide having at least 90% sequence identity to the mature polypeptide of SEQ ID NO:
- 2 or a GH61 polypeptide comprising the mature polypeptide of SEQ ID NO:
- 4;
- (b) a GH61 polypeptide encoded by a polynucleotide that hybridizes under at least very high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof, wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 0.2×SSC, 0.2% SDS at 70° C.;
- (c) a GH61 polypeptide encoded by a polynucleotide having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof, or a GH61 polypeptide encoded by a polynucleotide comprising the mature polypeptide coding sequence of SEQ ID NO:
- 3;
- and
- (d) a fragment of the GH61 polypeptide of the mature polypeptide of SEQ ID NO:
- 2 that has cellulolytic enhancing activity.
9 more elements
A process for producing a fermentation product, comprising:
- (a) saccharifying a cellulosic material with an enzyme composition comprising a GH61 polypeptide having cellulolytic enhancing activity, wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from the group consisting of:
- (i) a GH61 polypeptide having at least 90% sequence identity to the mature polypeptide of SEQ ID NO:
- 2 or a GH61 polypeptide comprising the mature polypeptide of SEQ ID NO:
- 4;
- (ii) a GH61 polypeptide encoded by a polynucleotide that hybridizes under at least very high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof, wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 0.2×SSC, 0.2% SDS at 70° C.;
- (iii) a GH61 polypeptide encoded by a polynucleotide having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof, or a GH61 polypeptide encoded by a polynucleotide comprising the mature polypeptide coding sequence of SEQ ID NO:
- 3;
- and
- (iv) a fragment of the GH61 polypeptide of the mature polypeptide of SEQ ID NO:
- 2 that has cellulolytic enhancing activity;
- (b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product;
- and
- (c) recovering the fermentation product from the fermentation.
12 more elements
A process of fermenting a cellulosic material, comprising:
- fermenting the cellulosic material with one or more fermenting microorganisms, wherein the cellulosic material is saccharified with an enzyme composition comprising a GH61 polypeptide having cellulolytic enhancing activity, wherein the GH61 polypeptide having cellulolytic enhancing activity is selected from the group consisting of:
- (a) a GH61 polypeptide having at least 90% sequence identity to the mature polypeptide of SEQ ID NO:
- 2 or a GH61 polypeptide comprising the mature polypeptide of SEQ ID NO:
- 4;
- (b) a GH61 polypeptide encoded by a polynucleotide that hybridizes under at least very high stringency conditions with the full-length complement of the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof, wherein very high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, and washing three times each for 15 minutes using 0.2×SSC, 0.2% SDS at 70° C.;
- (c) a GH61 polypeptide encoded by a polynucleotide having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof, or a GH61 polypeptide encoded by a polynucleotide comprising the mature polypeptide coding sequence of SEQ ID NO:
- 3;
- and
- (d) a fragment of the GH61 polypeptide of the mature polypeptide of SEQ ID NO:
- 2 that has cellulolytic enhancing activity.
9 more elements
Dependencies are inferred from the claim text.