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US 9,765,220 B2Granted
Water soluble fluorescent or colored dyes and methods for their use
AssigneesSony Corporation, Sony Corporation of America
InventorsTracy Matray, Hesham Sherif, C. Frederick Battrell
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Abstract
Compounds useful as fluorescent or colored dyes are disclosed. The compounds have the following structure (I): including stereoisomers, salts and tautomers thereof, wherein R1, R2, R3, L1, L2, L3, L4, L5, L6, M1, M2, A, q, w and n are as defined herein. Methods associated with preparation and use of such compounds are also provided.
Claims27
A compound having the following structure (III):
- wherein:
- M1 and M2 are, at each occurrence, independently a moiety comprising two or more double bonds and at least one degree of conjugation, and at least one occurrence of M1 is a moiety comprising three or more aryl or heteroaryl rings, or combinations thereof;
- L1, L3, L4 L6, L7 and L8 are, at each occurrence, independently optional alkylene or heteroalkylene linkers;
- R1 is, at each occurrence, independently H, alkyl or alkoxy;
- R2 is an electron pair, H, alkyl, alkylether, hydroxylalkyl, aminoalkyl, hydroxylalkylether, sulfhydrylalkyl, sulfyhdrylalkylether, cyanoalkyl, phospho, thiophospho, alkylphospho, alkylthiophospho, alkyletherphospho, alkyletherthiophospho, phosphoalkyl, phosphoalkylether, thiophosphoalkyl or thiophosphoalkylether, or R2 is a linker comprising a covalent bond to a biomolecule or microparticle;
- R3 is H, OH, SH, —NH2, alkyl, alkylether, hydroxylalkyl, aminoalkyl, hydroxylalkylether, sulfhydrylalkyl, sulfyhdrylalkylether, cyanoalkyl, —Oaralkyl, phosphate, thiophosphate, alkylphospho, alkylthiophospho, —Oalkylphospho, —Oalkylthiophospho, alkyletherphospho, alkyletherthiophospho, —Oalkyletherphospho, —Oalkyletherthiophospho phosphoalkyl, phosphoalkylether, thiophosphoalkyl, thiophosphoalkylether, —Ophosphoalkyl, O-phosphoalkylether, —Othiophosphoalkyl or —Othiophosphoalkylether;
- R4 is, at each occurrence, independently O−, S−, OZ, SZ or N(R6)2, where Z is a cation and each R6 is independently H or alkyl;
- R5 is, at each occurrence, independently oxo, thioxo or absent;
- n is an integer from 2 to 20;
- and
- q and w are each independently 0 or 1 for each integral value of n, wherein q is 1 for at least two integral values of n, or wherein q and w are each independently one for at least one integral value of n.
8 more elements
A compound having one of the following structures:
Dependencies are inferred from the claim text.