US 9,765,135 B2 · Anti-C5 antibodies

Abstract

An objective of the invention is to provide anti-C5 antibodies and methods of using the same. The invention provides anti-C5 antibodies and methods of using the same. In some embodiments, an isolated anti-C5 antibody of the present invention binds to an epitope within the β chain of C5 with a higher affinity at neutral pH than at acidic pH. The invention also provides isolated nucleic acids encoding an anti-C5 antibody of the present invention. The invention also provides host cells comprising a nucleic acid of the present invention. The invention also provides a method of producing an antibody comprising culturing a host cell of the present invention so that the antibody is produced. The invention further provides a method of producing an anti-C5 antibody comprising immunizing an animal against a polypeptide which comprises the MG1-MG2 domain of the β chain of C5. Anti-C5 antibodies of the present invention may be for use as a medicament. Anti-C5 antibodies of the present invention may be for use in treating a complement-mediated disease or condition which involves excessive or uncontrolled activation of C5. Anti-C5 antibodies of the present invention may be for use in enhancing the clearance of C5 from plasma.

Claims66

Dependent claims

An isolated antibody that binds to C5, wherein the antibody comprises:

(a) a HVR-H1 comprising the amino acid sequence SSYYX1X2, wherein X1 is M or V, X2 is C or A (SEQ ID NO:
126);
(b) a HVR-H2 comprising the amino acid sequence X1IX2TGSGAX3YX4AX5WX6KG, wherein X1 is C, A or G, X2 is Y or F, X3 is T, D or E, X4 is Y, K or Q, X5 is S, D or E, X6 is A or V (SEQ ID NO:
127);
and
(c) a HVR-H3 comprising the amino acid sequence DX1GYX2X3PTHAMX4X5, wherein X1 is G or A, X2 is V, Q or D, X3 is T or Y, X4 is Y or H, X5 is L or Y (SEQ ID NO:
128);
(d) a HVR-L1 comprising the amino acid sequence X1ASQX2IX3SX4LA, wherein X1 is Q or R, X2 is N, Q or G, X3 is G or S, X4 is D, K or S (SEQ ID N:
129);
(e) a HVR-L2 comprising the amino acid sequence GASX1X2X3S, wherein X1 is K, E or T, X2 is L or T, X3 is A, H, E or Q (SEQ ID NO:
130);
and
(f) a HVR-L3 comprising the amino acid sequence QX1TX2VGSSYGNX3, wherein X1 is S, C, N or T, X2 is F or K, X3 is A, T or H (SEQ ID NO:
131).

11 more elements

An isolated antibody that binds to C5, wherein the antibody comprises:

(a) (i) a HVR-H1 comprising the amino acid sequence of SEQ ID NO:
117;
(ii) a HVR-H2 comprising the amino acid sequence of SEQ ID NO:
119;
(iii) a HVR-H3 comprising the amino acid sequence of SEQ ID NO:
121;
(iv) a HVR-L1 comprising the amino acid sequence of SEQ ID NO:
122;
(v) a HVR-L2 comprising the amino acid sequence of SEQ ID NO:
123;
and
(vi) a HVR-L3 comprising the amino acid sequence of SEQ ID NO:
125;
(b) (i) a HVR-H1 comprising the amino acid sequence of SEQ ID NO:
117;
(ii) a HVR-H2 comprising the amino acid sequence of SEQ ID NO:
118;
(iii) a HVR-H3 comprising the amino acid sequence of SEQ ID NO:
121;
(iv) a HVR-L1 comprising the amino acid sequence of SEQ ID NO:
122;
(v) a HVR-L2 comprising the amino acid sequence of SEQ ID NO:
124;
and
(vi) a HVR-L3 comprising the amino acid sequence of SEQ ID NO:
125;
(c) (i) a HVR-H1 comprising the amino acid sequence of SEQ ID NO:
117;
(ii) a HVR-H2 comprising the amino acid sequence of SEQ ID NO:
120;
(iii) a HVR-H3 comprising the amino acid sequence of SEQ ID NO:
121;
(iv) a HVR-L1 comprising the amino acid sequence of SEQ ID NO:
122;
(v) a HVR-L2 comprising the amino acid sequence of SEQ ID NO:
124;
and
(vi) a HVR-L3 comprising the amino acid sequence of SEQ ID NO:
125;
and
(d) (i) a HVR-H1 comprising the amino acid sequence of SEQ ID NO:
54;
(ii) a HVR-H2 comprising the amino acid sequence of SEQ ID NO:
64;
(iii) a HVR-H3 comprising the amino acid sequence of SEQ ID NO:
74;
(iv) a HVR-L1 comprising the amino acid sequence of SEQ ID NO:
84;
(v) a HVR-L2 comprising the amino acid sequence of SEQ ID NO:
94;
and
(vi) a HVR-L3 comprising the amino acid sequence of SEQ ID NO:
104.

50 more elements

Dependencies are inferred from the claim text.

Anti-C5 antibodies

Life of this patent

Anti-C5 antibodies

Life of this patent

Abstract

Claims66