Methods for production of platelets from pluripotent stem cells and compositions thereof
AssigneeAstellas Institute for Regenerative Medicine
InventorsQiang Feng, Shi-Jiang Lu, Robert P. Lanza
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Abstract
Methods for production of platelets from pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) are provided. These methods may be performed without forming embryoid bodies or clusters of pluripotent stem cells, and may be performed without the use of stromal inducer cells. Additionally, the yield and/or purity can be greater than has been reported for prior methods of producing platelets from pluripotent stem cells. Also provided are compositions and pharmaceutical preparations comprising platelets, preferably produced from pluripotent stem cells.
Claims19
A method for producing platelets from a cell population comprising megakaryocytes, comprising:
- contacting a feeder-free, non-adherent culture that includes a cell population comprising megakaryocytes positive for CD41a and CD42b expression with
- (i) TPO or a TPO agonist or
- (ii) hematopoietic expansion medium and optionally
- (1) TPO or a TPO agonist, SCF, IL-6 and IL-9 or
- (2) TPO or a TPO agonist, SCF, and IL-11
- to cause the formation in culture of proplatelets that release platelets,
- at least 60% of which are positive for CD41a and CD42b expression.
Dependencies are inferred from the claim text.