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US 9,758,823 B2Granted
Enzyme method
AssigneeOxford Nanopore Technologies PLC
InventorsRuth Moysey, Andrew John Heron
Life of this patent
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Abstract
The invention relates to a new method of characterizing a target polynucleotide. The method uses a pore and a Hel308 helicase or amolecular motor which is capable of binding to the target polynucleotide at an internal nucleotide. The helicase or molecular motor controls the movement of the target polynucleotide through the pore.
Claims11
A method of characterising a target polynucleotide, comprising:
- (a) providing in an aqueous solution a transmembrane pore and a membrane, wherein the transmembrane pore is present in the membrane, and wherein the aqueous solution comprises a salt at a concentration in a range of 0.3 M to 3 M;
- (b) combining, in the aqueous solution of step (a), the target polynucleotide and a Hel308 helicase, wherein the helicase binds to the target polynucleotide to form a polynucleotide helicase complex, thereby controlling the movement of the target polynucleotide through the transmembrane pore;
- and
- (c) measuring, during an application of a potential across the transmembrane pore, an ionic current as the target polynucleotide moves through the transmembrane pore, to determine one or more characteristics of the target polynucleotide and thereby characterising the target polynucleotide.
1 more element
Dependencies are inferred from the claim text.