Porous polysaccharide scaffold comprising nano-hydroxyapatite and use for bone formation
AssigneesInstitut National De La Sante Et De La Recherche Medicale (Inserm), Universite de Bordeaux II Victor Segalen, Universite Paris Diderot—Paris 7
InventorsJoelle Amedee, Didier Letourneur, Catherine Le Visage, Sidi Mohammed Derkaoui, Jean-Christophe Fricain, Sylvain Catros
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Abstract
The present invention relate to three dimensional porous polysaccharide matrices able to induce mineralisation of a tissue in osseous site, as well as in non-osseous site, in the absence of stent cells or growth factors.
Claims20
A method for bone generation at an osseous or a non-osseous site, comprising the step of administering, at the osseous or the non-osseous site, a porous polysaccharide scaffold obtained by
- i) preparing an alkaline aqueous solution comprising at least one polysaccharide, a cross-linking agent and a porogen agent,
- ii) transforming the solution into a hydrogel by placing said solution at a temperature from 4° C. to 80° C. for a sufficient time to allow cross-linking of said at least one polysaccharide,
- iii) submerging said hydrogel into a solvent, and
- iv) washing the porous polysaccharide scaffold obtained at step iii), wherein the alkaline aqueous solution of step i) further comprises hydroxyapatite, and wherein the step of administering is performed in the absence of cells and growth factors.
A method for bone generation at an osseous or a non-osseous site comprising the step of administering, at the osseous or the non-osseous site, a porous polysaccharide scaffold obtained by:
- a) preparing an alkaline aqueous solution comprising at least one polysaccharide, and one cross-linking agent,
- b) freezing the aqueous solution of step a), and
- c) sublimating the frozen solution of step b),
- wherein the alkaline aqueous solution of step a) further comprises hydroxyapatite,
- and wherein step b) is performed before cross-linking of the polysaccharide occurs in the solution of step a),
- and wherein the step of administering is performed in the absence of cells and growth factors.
Dependencies are inferred from the claim text.