# US 9,753,145 B2 · Dosimetric method > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 9,753,145 B2 - Document ID: US9753145B2 - Status: Granted - Kind: B2 - Application number: 15/029,429 - Filed: 2014-10-28 - Published: 2016-08-11 - Granted: 2017-09-05 - Earliest claimed: 2013-10-28 - Assignees: Nitto Denko Corporation - Applicants: Nitto Denko Corporation - Inventors: Geofrey De Visscher; Nicolas Schüwer - Web page: https://patentgrep.com/patent/US9753145B2 ## CPC - G01T 1/04: Chemical dosimeters (G01T1/06, G01T1/08 take precedence) - G01T: MEASUREMENT OF NUCLEAR OR X-RADIATION (radiation analysis of materials, mass spectrometry G01N23/00; tubes for determining the presence, intensity, density or energy of radiation or particles H01J47/00) ## Abstract The present invention relates to a method for detecting and optionally quantifying exposure to ionizing radiation and applications thereof. More particularly, the invention relates to the detection and/or quantification of ionizing radiation exposure by detecting the colour change of one or more dianhydrohexitol compounds, such as isosorbide, caused by ionizing radiation. It also relates to the use of dianhydrohexitol compounds for detecting exposure to irradiation, to a system and kit that may be used for such purposes, and to a method for making sterilized objects, which also relies on the colour change induced by ionizing radiation. ## Claims ### Claim 1 A method for detecting and optionally quantifying exposure to ionizing radiation, comprising the steps: (a) providing a dianhydrohexitol or dianhydrohexitol-containing composition, wherein the dianhydrohexitol is one or more of isosorbide, isomannide and isoidide; (b) exposing the dianhydrohexitol or dianhydrohexitol-containing composition to ionizing radiation, thereby effecting a colour change in the dianhydrohexitol; (c) detecting the colour change in the dianhydrohexitol or dianhydrohexitol-containing composition; and optionally (d) quantifying the dose of ionizing radiation absorbed by the dianhydrohexitol or dianhydrohexitol-containing composition on the basis of the colour change. ### Claim 2 (depends on 1) The method according to claim 1, wherein detecting the colour change is performed visually and the method further comprises the step of quantifying the dose of ionizing radiation by comparing the colour change with a reference to determine (i) if a pre-defined minimum exposure has occurred and optionally (ii) the quantity of exposure that has occurred. ### Claim 3 (depends on 2) The method according to claim 2, wherein the exposed dianhydrohexitol or dianhydrohexitol-containing composition is dissolved in a solvent and the step of detecting the colour change is performed on a solution of the exposed dianhydrohexitol or dianhydrohexitol-containing composition. ### Claim 4 (depends on 2) The method according to claim 2, wherein the dianhydrohexitol is isosorbide. ### Claim 5 (depends on 1) The method according to claim 1, wherein detecting the colour change is performed with a colorimetric , chromometric or spectrometric device and the method further comprises the step of quantifying the dose of ionizing radiation by comparing the colour change with a reference to determine (i) if a pre-defined minimum exposure has occurred and optionally (ii) the quantity of exposure that has occurred. ### Claim 6 (depends on 5) The method according to claim 5, wherein the exposed dianhydrohexitol or dianhydrohexitol-containing composition is dissolved in a solvent and the step of detecting the colour change is performed on a solution of the exposed dianhydrohexitol or dianhydrohexitol-containing composition. ### Claim 7 (depends on 5) The method according to claim 5, wherein the dianhydrohexitol is isosorbide. ### Claim 8 (depends on 5) The method according to claim 5, wherein the colorimetric device is a UV-vis spectrometer. ### Claim 9 (depends on 8) The method according to claim 8, wherein the exposed dianhydrohexitol or dianhydrohexitol-containing composition is dissolved in a solvent and the step of detecting the colour change is performed on a solution of the exposed dianhydrohexitol or dianhydrohexitol-containing composition. ### Claim 10 (depends on 8) The method according to claim 8, wherein the dianhydrohexitol is isosorbide. ### Claim 11 (depends on 1) The method according to claim 1, wherein the exposed dianhydrohexitol or dianhydrohexitol-containing composition is dissolved in a solvent and the step of detecting the colour change is performed on a solution of the exposed dianhydrohexitol or dianhydrohexitol-containing composition. ### Claim 12 (depends on 11) The method according to claim 11, wherein the dianhydrohexitol is isosorbide. ### Claim 13 (depends on 1) The method according to claim 1, wherein the dianhydrohexitol is isosorbide. ### Claim 14 (depends on 1) The method according to claim 1, wherein the ionizing radiation is X-ray or γ-ray. ### Claim 15 A method for detecting and optionally quantifying exposure to ionizing radiation, comprising the steps: (a) providing a dianhydrohexitol or dianhydrohexitol-containing composition, wherein the dianhydrohexitol is one or more of isosorbide, isomannide and isoidide; (b) exposing the dianhydrohexitol or dianhydrohexitol-containing composition to ionizing radiation, thereby producing a free radical; (c) detecting a free radical in the dianhydrohexitol or dianhydrohexitol-containing composition by electron paramagnetic resonance (EPR); and optionally (d) quantifying the dose of ionizing radiation absorbed by the dianhydrohexitol or dianhydrohexitol-containing composition on the basis of the EPR signal. ### Claim 16 (depends on 15) The method according to claim 15, wherein the dianhydrohexitol is isosorbide. ### Claim 17 A system for detecting and optionally quantifying exposure to ionizing radiation comprising: a dianhydrohexitol; and a means to determine if (i) the dianhydrohexitol has been exposed to a pre-defined dose of ionizing radiation and optionally (ii) the quantity of the exposure that has occurred. ### Claim 18 A kit comprising: a dianhydrohexitol or dianhydrohexitol-containing composition, wherein the dianhydrohexitol is one or more of isosorbide, isomannide and isoidide; and a means to determine if (i) the dianhydrohexitol or dianhydrohexitol-containing composition has been exposed to a pre-defined dose of ionizing radiation and optionally (ii) the quantity of the exposure that has occurred. ### Claim 19 (depends on 18) A kit according to claim 18, which is for determining whether an object has been effectively sterilized, wherein the means is a colour chart and pre-defined dose of ionizing radiation is the minimum dosage required for effective sterilization. ### Claim 20 (depends on 19) A kit according to claim 19, wherein the pre-defined dose of ionizing radiation is 5 kGy or higher, preferably 20 kGy or higher, more preferably 25 kGy or higher.