Method and devices for applying substances to a support, especially monomers for the combinatorial synthesis of molecule libraries
AssigneesDeutsches Krebsforschungszentrum Stiftung des Offentlichen Rechts, Europaisches Laboratorium fur Molekularbiologie
InventorsAnnemarie Poustka, Frank Breitling, Karl-Heinz Gross, Stefan Dübel, Rainer Saffrich
Life of this patent
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Abstract
For the combinatorial synthesis of molecule libraries, substances are embedded in a matrix consisting of a first solvent thereby forming transport units in a solid state of aggregation at a temperature of less than 90° C. and wherein after application to a support, the physical environment of the transport units is modified by the application of a physical process such as a laser printer whereby the substances in the transport units are linked to the support.
Claims16
A method for applying substances including monomers to a support for the combinatorial synthesis of molecule libraries, comprising the steps of:
- embedding at least one amino acid monomer or nucleotide monomer into a matrix of at least one solvent that at a temperature of less than 90° C. is in a solid state of aggregation, thereby forming monomer-immobilizing transport units;
- applying these transport units in the solid state of aggregation onto a solid support by laser printing at a temperature of less than 90° C., whereby the transport units become electrostatically charged and thereby transferred onto pre-determined regions of a laser-writable roller and thereafter applied to the support, where the transport units are remaining in the solid state of aggregation;
- thereafter, changing the transport units from a solid state of aggregation to a liquid state of aggregation thereby mobilizing the monomers and diffusing the monomers within the transport units, and
- thereafter, covalently linking the thus mobilized monomers to molecules located on the support through a linking reaction, thereby yielding a number of different monomers coupled to the support in the pre-determined locations and washing away non-linked monomers
- applying in this manner more than one layer of monomers to the support, whereby monomers from a second layer are covalently linked to monomers from a first layer that were previously linked to the support and washing away the non-linked monomers.
A method for applying substances to a support for the combinatorial synthesis of molecule libraries, comprising the steps of:
- embedding amino acid or nucleotide monomers into a matrix that includes at least one solvent at a temperature of less than 90° C. at a solid state of aggregation thereby forming toner particles that serve as transport units, said toner particles differ from each other by the monomers immobilized within;
- electrostatically charging said toner particles and positioning by laser printing, at different times, the toner particles in predetermined position to a solid support, whereby the toner particles remain in a solid state of aggregation and the monomers within the toner particles are temporarily blocked from coupling to the support;
- changing the toner particles from a solid state of aggregation to a liquid state of aggregation, thereby mobilizing the monomers and diffusing the monomers within the toner particles and thus permitting release of the monomers onto the support;
- covalently linking the thus released monomers to molecules located on the support through a linking reaction, thereby yielding a number of different monomers coupled to the support,
- wherein more than one layer of monomers is applied repeatedly one after the other to the support in defined positions, in each case followed by the covalent linking of the substances to the support and washing away non-linked substances.
Dependencies are inferred from the claim text.