Non-replicative transduction particles and transduction particle-based reporter systems
AssigneeGeneWeave Biosciences, Inc.
InventorsDiego Ariel Rey, Nikol de Forest, Heather Cox, Soni Shukla
Life of this patent
Estimating the term…
Abstract
Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules.
Claims24
A cell packaging system for packaging a reporter nucleic acid molecule into a non-replicative transduction particle, said cell packaging system comprising:
- a host cell;
- a virus nucleic acid molecule inside said host cell comprising a first gene that contains a first component being disrupted, wherein disruption of said first component prevents packaging of said virus nucleic acid molecule into said non-replicative transduction particle, wherein said disruption is selected from introducing a silent mutation into said first component or deleting said first component;
- and
- a reporter nucleic acid molecule inside said host cell and separate from said virus nucleic acid molecule, comprising a second gene that contains a second component, wherein said second component complements said disruption of said first component and facilitates the packaging of said reporter nucleic acid molecule into said non-replicative transduction particle.
A method for producing a non-replicative transduction particle packaged with a reporter nucleic acid, comprising:
- providing a host cell comprising a virus nucleic acid molecule inside said host cell comprising a first gene that contains a first component being disrupted, wherein disruption of said first component prevents packaging of said virus nucleic acid molecule into said non-replicative transduction particle, wherein said disruption is selected from introducing a silent mutation into said first component or deleting said first component;
- and a reporter nucleic acid molecule inside said host cell and separate from said virus nucleic acid molecule, comprising a second gene that contains a second component, wherein said second component complements said disruption of said first component and facilitates the packaging of said reporter nucleic acid molecule into said non-replicative transduction particle;
- inducing a lytic phase of said virus nucleic acid molecule to produce non-replicative transduction particles packaged with said reporter nucleic acid molecule;
- and
- isolating said non-replicative transduction particle.
Dependencies are inferred from the claim text.