# US 9,752,199 B2 · Biosensor system for the rapid detection of analytes

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- Number: US 9,752,199 B2
- Document ID: US9752199B2
- Status: Granted
- Kind: B2
- Application number: 15/086,769
- Filed: 2016-03-31
- Published: 2016-10-06
- Granted: 2017-09-05
- Earliest claimed: 2015-03-31
- Assignees: Fundamental Solutions Corporation
- Applicants: Fundamental Solutions Corporation
- Inventors: Thomas J. Zupancic; Lingchun Zeng; Srikanth Vedamoorthy; Joel S. Lwande; Joesph D. Kittle; Min Mo
- Web page: https://patentgrep.com/patent/US9752199B2

## CPC

- C12Q 1/6897: involving reporter genes operably linked to promoters
- C07K 14/315: from Streptococcus (G), e.g. Enterococci
- C07K 14/36: from Actinomyces; from Streptomyces (G)
- C07K 14/7051: {T-cell receptor (TcR)-CD3 complex}
- C07K 14/70535: {Fc-receptors, e.g. CD16, CD32, CD64 (CD2314/705F)}
- C07K 16/12: against material from bacteria
- G01N 33/5047: {Cells of the immune system}
- G01N 33/536: with immune complex formed in liquid phase
- G01N 33/56916: {Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia}
- C07K 2317/52: Constant or Fc region; Isotype
- C07K 2319/22: containing a Strep-tag
- C07K 2319/60: containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
- G01N 2333/245: Escherichia (G)
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)

## Abstract

A system, device, and method for rapid detection of analytes that includes a living, engineered biosensor cell that is typically a component of the mammalian immune system; a reporter protein that is engineered into and expressed by the living, engineered biosensor cell, wherein the reporter protein emits a detectable signal in response to certain predetermined changes in the cytosol of the living, engineered cell; a signal transduction pathway expressed by the living, engineered biosensor cell, wherein the signal transduction pathway controls a biological process within the cytosol of the living, engineered biosensor cell, and wherein the biochemical process, when it occurs, causes the reporter protein to emit a detectable signal; at least one type of detector molecule that is adapted to bind to a specific analyte; at least one analyte that binds to the detector molecule that is specific to that analyte; a plurality of non-antibody signal transducing elements that are either expressed by the living, engineered biosensor cell or that actively bind to a receptor or a receptor component expressed by the living, engineered biosensor cell, wherein each signal transducing element is adapted to receive a detector molecule.

## Claims

### Claim 1

A chimeric fusion protein having an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 4, 6, 6, 10, 12, and 18.

### Claim 2

A chimeric fusion protein having an amino acid sequence selected from the group consisting of SEQ ID NOS: 14 and 16.