# US 9,752,198 B2 · Corn event MIR162

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- Number: US 9,752,198 B2
- Document ID: US9752198B2
- Status: Granted
- Kind: B2
- Application number: 13/939,954
- Filed: 2013-07-11
- Published: 2014-06-12
- Granted: 2017-09-05
- Earliest claimed: 2006-06-03
- Assignees: Syngenta Participations AG
- Applicants: Syngenta Participations AG
- Inventors: Nykoll Long; Jeffrey Bottoms; Moez Rajabali Meghji; Hope Hart; Qiudeng Que; Derrick Pulliam
- Web page: https://patentgrep.com/patent/US9752198B2

## CPC

- C12Q 1/6895: for plants, fungi or algae
- C07K 14/415: from plants
- C12N 15/8286: {for insect resistance}
- C12Q 2600/13: Plant traits
- Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

A novel transgenic corn event designated MIR162 is disclosed. The invention relates to nucleic acids that are unique to event MIR162 and to methods for detecting the presence of the MIR162 event based on DNA sequences of the recombinant constructs inserted into the corn genome that resulted in the MIR162 event and of genomic sequences flanking the insertion site. The invention further relates to corn plants comprising the transgenic genotype of MIR162 and to methods for producing a corn plant by crossing a corn plant comprising the MIR162 genotype with itself or another corn variety. Seeds of corn plants comprising the MIR162 genotype are also objects of the present invention. The invention also relates to methods of controlling insects using MIR162 corn plants.

## Claims

### Claim 1

A method of detecting the presence of a nucleic acid molecule that is unique to event MIR162 in a sample comprising corn nucleic acids, the method comprising:
(a) contacting the sample with a pair of primers that, when used in a nucleic-acid amplification reaction with genomic DNA from event MIR162 produces an amplicon that is diagnostic for event MIR162;
(b) performing a nucleic acid amplification reaction, thereby producing the amplicon; and
(c) detecting the amplicon.

### Claim 2

A method of detecting the presence of a nucleic acid molecule that is unique to event MIR162 in a sample comprising corn nucleic acids, the method comprising:
(a) contacting the sample with a probe that hybridizes under high stringency conditions with genomic DNA from event MIR162 and does not hybridize under high stringency conditions with DNA of a control corn plant, wherein the probe comprises the nucleotide sequence of SEQ ID NO: 45 or SEQ ID NO: 47;
(b) subjecting the sample and probe to high stringency hybridization conditions; and
(c) detecting hybridization of the probe to the nucleic acid molecule.

### Claim 3

An amplicon diagnostic for event MIR162, wherein said amplicon is at least 20 nucleotides in length and comprises the nucleic acid sequence of SEQ ID NO: 45, SEQ ID NO: 47, the nucleotide of position 387 of SEQ ID NO: 1 or the nucleotide of position 1683 of SEQ ID NO: 1.

### Claim 4

A pair of polynucleotide primers comprising a first polynucleotide primer and a second polynucleotide primer which function together in the presence of an event MIR162 DNA template in a sample to produce an amplicon diagnostic for event MIR162, wherein said first primer and second primer comprise at least 10 contiguous nucleotides of SEQ ID NO: 1 and wherein said amplicon is at least 20 nucleotides in length and comprises the nucleotide of position 387 of SEQ ID NO: 1 and/or position 1683 of SEQ ID NO: 1.

### Claim 5 (depends on 4)

A method of detecting the presence of a nucleic acid molecule that is unique to event MIR162 in a sample comprising corn nucleic acids, the method comprising:
(a) contacting the sample with a pair of primers of claim 4 that, when used in a nucleic-acid amplification reaction with genomic DNA from event MIR162 produces an amplicon that is diagnostic for event MIR162;
(b) performing a nucleic acid amplification reaction, thereby producing the amplicon; and
(c) detecting the amplicon.