# US 9,752,185 B2 · Microfluidic devices

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- Number: US 9,752,185 B2
- Document ID: US9752185B2
- Status: Granted
- Kind: B2
- Application number: 14/824,333
- Filed: 2015-08-12
- Published: 2016-02-25
- Granted: 2017-09-05
- Earliest claimed: 2004-09-15
- Assignees: IntegenX Inc.
- Applicants: IntegenX Inc.
- Inventors: Allen Boronkay; Stevan Bogdan Jovanovich; Iuliu Ioan Blaga
- Web page: https://patentgrep.com/patent/US9752185B2

## CPC

- C12Q 1/6869: Methods for sequencing
- B01F 31/65: the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
- B01F 33/30: Micromixers
- B01L 3/5027: {by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip}
- B01L 3/502715: {characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces}
- B01L 3/502753: {characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation}
- C12N 15/1003: {Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor}
- C12N 15/1013: {by using magnetic beads}
- C12Q 1/24: Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
- C12Q 1/6806: Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence)
- C12Q 1/6844: Nucleic acid amplification reactions
- C12Q 1/686: Polymerase chain reaction [PCR]
- C12Q 1/70: involving virus or bacteriophage {(immunoassay for viruses G01N33/56983)}
- B01L 3/5025: {for parallel transport of multiple samples}
- B01L 3/502738: {characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746)}
- B01L 3/502761: {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules}
- B01L 7/52: {with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples}
- B01L 9/527: {for microfluidic devices, e.g. used for lab-on-a-chip}
- B01L 2200/027: for microfluidic devices
- B01L 2200/028: Modular arrangements
- B01L 2200/0668: Trapping microscopic beads
- B01L 2200/10: Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
- B01L 2300/0627: Sensor or part of a sensor is integrated
- B01L 2300/0636: Integrated biosensor, microarrays
- B01L 2300/0654: Lenses; Optical fibres
- B01L 2300/0803: Disc shape
- B01L 2300/0816: Cards, e.g. flat sample carriers usually with flow in two horizontal directions
- B01L 2300/0864: comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
- B01L 2300/0867: Multiple inlets and one sample wells, e.g. mixing, dilution
- B01L 2300/087: Multiple sequential chambers
- B01L 2300/0874: Three dimensional network
- B01L 2300/0887: Laminated structure
- B01L 2300/1822: using Peltier elements
- B01L 2300/1827: using resistive heater
- B01L 2300/1844: using fans
- B01L 2400/0415: electrical forces, e.g. electrokinetic
- B01L 2400/0421: electrophoretic flow
- B01L 2400/0481: squeezing of channels or chambers
- B01L 2400/0655: pinch valves
- G01N 2035/00514: {Stationary mixing elements}
- Y10T 436/163333: Organic [e.g., chemical warfare agents, insecticides, etc.]
- Y10T 436/25875: Gaseous sample or with change of physical state
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- B01F: MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING (mixing paints B44D3/06)
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- Y10T: TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

## Abstract

Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense. Also disclosed in the present disclosure is a flow through, traveling-wave, bead-beating device which comprises a rotating pole piece, a flow through tube, and a magnetic piece. Rotation of the rotating pole piece may create a magnetic wave down the flow through tube, thereby producing sufficient acceleration of beads through the tube to disrupt or lyse target analytes flowing through the tube.

## Claims

### Claim 1

A traveling magnetic wave flow through device, comprising:
a rotating pole piece;
a flow through tube;
a magnetic fixed piece; and
at least one bead positioned in the lumen of said flow through tube, wherein said flow through tube is in fluidic communication with a microfluidic device, and wherein said rotating pole piece is configured to produce a travelling magnetic wave down said flow through tube upon rotation of said rotating pole piece.

### Claim 2 (depends on 1)

The device of claim 1, wherein the rotation of said rotating pole piece is at least about 100 Hz.

### Claim 3 (depends on 1)

The device of claim 1, wherein said flow through tube is adjacent to said rotating pole piece.

### Claim 4 (depends on 3)

The device of claim 3, wherein said flow through tube is axially parallel to said rotating pole piece.

### Claim 5 (depends on 3)

The device of claim 3, wherein said flow through tube is fluidically separated from said rotating pole piece.

### Claim 6 (depends on 1)

The device of claim 1, further comprising wherein said at least one bead comprises a plurality of beads positioned in the lumen of said flow through tube.

### Claim 7 (depends on 6)

The device of claim 6, wherein said plurality of beads comprises magnetic beads.

### Claim 8 (depends on 7)

The device of claim 7, wherein said rotation of said rotating pole piece produces acceleration of said magnetic beads to lyse target analytes flowing through said flow through tube.